Overview

  • Product nameAnti-SHFM3 antibody
    See all SHFM3 primary antibodies
  • Description
    Rabbit polyclonal to SHFM3
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    C-terminal synthetic peptide (Human) conjugated to KLH

  • Positive control
    • IHC-P: Human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab60116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • FunctionProbably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Likely to be involved in key signaling pathways crucial for normal limb development. May participate in Wnt signaling.
  • Tissue specificityExpressed in brain, kidney, lung and liver.
  • Involvement in diseaseDefects in FBXW4 are a cause of split-hand/foot malformation type 3 (SHFM3) [MIM:246560]. SHFM3 is an autosomal dominant disorder characterized by hypoplasia/aplasia of the central digits with fusion of the remaining digits.
  • Sequence similaritiesContains 1 F-box domain.
    Contains 4 WD repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAC antibody
    • Dactylin antibody
    • F box and WD 40 domain containing protein 4 antibody
    • F box and WD 40 domain protein 4 antibody
    • F box and WD repeat domain containing 4 antibody
    • F box/WD repeat containing protein 4 antibody
    • F box/WD repeat protein 4 antibody
    • F-box and WD-40 domain-containing protein 4 antibody
    • F-box/WD repeat-containing protein 4 antibody
    • FBW 4 antibody
    • FBW4 antibody
    • FBWD 4 antibody
    • FBWD4 antibody
    • FBXW 4 antibody
    • FBXW4 antibody
    • FBXW4_HUMAN antibody
    • OTTHUMP00000059175 antibody
    • SHFM 3 antibody
    • SHSF 3 antibody
    • SHSF3 antibody
    • Split hand/foot malformation (ectrodactyly) type antibody
    see all

Anti-SHFM3 antibody images

  • ab60116, at 1/100 dilution, staining SHFM3 in formalin-fixed, paraffin-embedded human hepatocarcinoma tissue by immunohistochemistry. ab60116 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References for Anti-SHFM3 antibody (ab60116)

ab60116 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293FT)
Loading amount 20 µg
Specification 293FT
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 10 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"