• Product nameAnti-Shigella antibody (Biotin)
    See all Shigella primary antibodies
  • Description
    Rabbit polyclonal to Shigella (Biotin)
  • ConjugationBiotin
  • SpecificityPolyvalent for Shigella antigens. Reacts with Shigella species including sonnei. Antiserum is not absorbed and may react with for related Enterobacteriaceae.
  • Tested applicationsSuitable for: ICC/IFmore details
  • Species reactivity
    Reacts with Shigella species including sonnei. Antiserum is not absorbed and may react with related Enterobacteriaceae. Not yet tested in other species.
  • Immunogen

    Mixture of S. boydii, S. flexneri and S. dysenteriae.

  • General notes

    Purified IgG fraction covalently coupled with N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab20296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIF: Use at an assay dependent dilution. Recommended for use with avidin and streptavidin amplification systems for fluorescence microscopy. Acetone fixation of the antigen source is recommended prior to staining. Spot suspension of bacteria onto slide. Air dry. Fix slide with 100% acetone for 5 minutes at room temperature. Air dry. Stain.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceShigella is a genus of Gram-negative, non-motile, non-spore forming rod-shaped bacteria closely related to Escherichia coli and Salmonella. The causative agent of human shigellosis, Shigella also cause disease in other primates, but not in other mammals. Shigella species are classified by four serogroups: * Serogroup A: S. dysenteriae (12 serotypes) * Serogroup B: S. flexneri (6 serotypes) * Serogroup C: S. boydii (23 serotypes) * Serogroup D: S. sonnei (1 serotype) Shigella infection causes dysentery that results in the destruction of the epithelial cells of the intestinal mucosa in the cecum and rectum. Some strains produce enterotoxin and Shiga toxin, similar to the verotoxin of E. coli O157:H7.
    • Alternative names
      • S. boydii antibody
      • S. dysenteriae antibody
      • S. flexneri antibody
      • S. sonnei antibody
      • Shigella Boydii antibody
      • Shigella Dysenteriae antibody
      • Shigella Flexneri antibody
      • Shigella Sonnei antibody
      see all

    References for Anti-Shigella antibody (Biotin) (ab20296)

    ab20296 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab20296.
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