Anti-Shigella antibody (HRP) (ab19952)


  • Product nameAnti-Shigella antibody (HRP)
    See all Shigella primary antibodies
  • Description
    Rabbit polyclonal to Shigella (HRP)
  • ConjugationHRP
  • SpecificityRabbit polyclonal to Shigella (HRP)(ab19952) is polyvalent for Shigella antigens. It reacts with Shigella species including sonnei. Antiserum is not absorbed.
  • Tested applicationsSuitable for: ICC/IFmore details
  • Species reactivity
    Reacts with related Enterobacteriaceae. Not yet tested in other species.
  • Immunogen

    Mixture of S. boydii (ATCC #8700), S. flexneri (ATCC #29903) and S. dysenteriae (ATCC #13313)

  • General notes

    Rabbit polyclonal to Shigella (HRP) consists of the purified IgG fraction covalently coupled with highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Estimated molar HRP: IgG substitution is 2-3.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.002% Thimerosal (merthiolate)
    Constituents: 10mg/ml BSA, 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesFree horseradish peroxidase enzyme is not present.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab19952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIF: Use at an assay dependent dilution.
    Acetone fixation of the antigen source is recommended prior to staining. Spot suspension of bacteria onto slide. Air dry. Fix slide with 100% Acetone for 5 minutes at room temperature. Air dry. Stain.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceShigella is a genus of Gram-negative, non-motile, non-spore forming rod-shaped bacteria closely related to Escherichia coli and Salmonella. The causative agent of human shigellosis, Shigella also cause disease in other primates, but not in other mammals. Shigella species are classified by four serogroups: * Serogroup A: S. dysenteriae (12 serotypes) * Serogroup B: S. flexneri (6 serotypes) * Serogroup C: S. boydii (23 serotypes) * Serogroup D: S. sonnei (1 serotype) Shigella infection causes dysentery that results in the destruction of the epithelial cells of the intestinal mucosa in the cecum and rectum. Some strains produce enterotoxin and Shiga toxin, similar to the verotoxin of E. coli O157:H7.
    • Alternative names
      • S. boydii antibody
      • S. dysenteriae antibody
      • S. flexneri antibody
      • S. sonnei antibody
      • Shigella Boydii antibody
      • Shigella Dysenteriae antibody
      • Shigella Flexneri antibody
      • Shigella Sonnei antibody
      see all

    References for Anti-Shigella antibody (HRP) (ab19952)

    ab19952 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    I am sorry to hear that you have been experiencing issues when using this product in ELISA. As stated on the datasheet of this product; Shigella is a genus of Gram-negative, non-motile, non-spore forming rod-sh...

    Read More

    Thank you for contacting Abcam.

    Please find attached the Certificate of Compliances for the 6 antibodies that you purchased recently.

    If there is anything else I can help you with, please let me know.