Overview

  • Product name
  • Description
    Rabbit polyclonal to SHIP
  • Specificity
    ab59338 detects endogenous levels of total SHIP protein.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized non-phosphopeptide derived from human SHIP around the phosphorylation site of tyrosine 1020 (P-L-YP-G-S).

  • Positive control
    • WB: Extracts from mouse brain cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
ELISA 1/5000.

Target

  • Function
    Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6.
  • Tissue specificity
    Specifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level).
  • Sequence similarities
    Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.
    Contains 1 SH2 domain.
  • Domain
    The SH2 domain interacts with tyrosine phosphorylated forms of proteins such as SHC1 or PTPN11/SHP-2. It competes with that of GRB2 for binding to phosphorylated SHC1 to inhibit the Ras pathway. It is also required for tyrosine phosphorylation.
    The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding.
  • Cellular localization
    Cytoplasm. Membrane. Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Partly translocated via its SH2 domain which mediates interaction with tyrosine phosphorylated receptors such as the FC-gamma-RIIB receptor (FCGR2B) or CD16/FCGR3. Tyrosine phosphorylation may also participate to membrane localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Inositol polyphosphate 5 phosphatase of 145kDa antibody
    • 4 antibody
    • 5-trisphosphate 5-phosphatase 1 antibody
    • hp51CN antibody
    • Inositol polyphosphate 5 phosphatase 145kDa antibody
    • Inositol polyphosphate 5 phosphatase antibody
    • Inositol polyphosphate-5-phosphatase of 145 kDa antibody
    • INPP5D antibody
    • MGC104855 antibody
    • MGC142140 antibody
    • MGC142142 antibody
    • p150Ship antibody
    • Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 1 antibody
    • Phosphatidylinositol-3 antibody
    • SH2 containing inositol phosphatase isoform b antibody
    • SH2 domain containing inositol 5' phosphatase 1 antibody
    • SH2 domain containing inositol phosphatase 1 antibody
    • SH2 domain-containing inositol phosphatase 1 antibody
    • SH2 domain-containing inositol-5''-phosphatase 1 antibody
    • SHIP-1 antibody
    • SHIP1 antibody
    • SHIP1_HUMAN antibody
    • Signaling inositol polyphosphate 5 phosphatase SIP 145 antibody
    • SIP-145 antibody
    • SIP145 antibody
    see all

Anti-SHIP antibody images

  • All lanes : Anti-SHIP antibody (ab59338) at 1/500 dilution

    Lane 1 : Mouse brain cells minus immunising peptide
    Lane 2 : Mouse brain cells plus immunising peptide


    Predicted band size : 133 kDa
    Observed band size : 133 kDa

References for Anti-SHIP antibody (ab59338)

ab59338 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Catfish Cell lysate - whole cell (clonal B cells, clonal cytotoxic T lymphocytes)
Specification
clonal B cells, clonal cytotoxic T lymphocytes
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 31 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up