Anti-SHIP (phospho Y1020) antibody [EPR425] (ab96402)


  • Product nameAnti-SHIP (phospho Y1020) antibody [EPR425]
    See all SHIP primary antibodies
  • Description
    Rabbit monoclonal [EPR425] to SHIP (phospho Y1020)
  • Tested applicationsSuitable for: WB, IP, ICCmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SHIP (phospho Y1020). The exact sequence is proprietary.

  • Positive control
    • WB: Raji whole cell lysate - treated with pervanadate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab96402 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 145 kDa (predicted molecular weight: 133 kDa).
IP 1/20.
ICC 1/250 - 1/500.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • FunctionPhosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6.
    • Tissue specificitySpecifically expressed in immune and hematopoietic cells. Expressed in bone marrow and blood cells. Levels vary considerably within this compartment. Present in at least 74% of immature CD34+ cells, whereas within the more mature population of CD33+ cells, it is present in only 10% of cells. Present in the majority of T-cells, while it is present in a minority of B-cells (at protein level).
    • Sequence similaritiesBelongs to the inositol-1,4,5-trisphosphate 5-phosphatase family.
      Contains 1 SH2 domain.
    • DomainThe SH2 domain interacts with tyrosine phosphorylated forms of proteins such as SHC1 or PTPN11/SHP-2. It competes with that of GRB2 for binding to phosphorylated SHC1 to inhibit the Ras pathway. It is also required for tyrosine phosphorylation.
      The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain.
    • Post-translational
      Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding.
    • Cellular localizationCytoplasm. Membrane. Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Partly translocated via its SH2 domain which mediates interaction with tyrosine phosphorylated receptors such as the FC-gamma-RIIB receptor (FCGR2B) or CD16/FCGR3. Tyrosine phosphorylation may also participate to membrane localization.
    • Information by UniProt
    • Database links
    • Alternative names
      • Inositol polyphosphate 5 phosphatase of 145kDa antibody
      • 4 antibody
      • 5-trisphosphate 5-phosphatase 1 antibody
      • hp51CN antibody
      • Inositol polyphosphate 5 phosphatase 145kDa antibody
      • Inositol polyphosphate 5 phosphatase antibody
      • Inositol polyphosphate-5-phosphatase of 145 kDa antibody
      • INPP5D antibody
      • MGC104855 antibody
      • MGC142140 antibody
      • MGC142142 antibody
      • p150Ship antibody
      • Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 1 antibody
      • Phosphatidylinositol-3 antibody
      • SH2 containing inositol phosphatase isoform b antibody
      • SH2 domain containing inositol 5' phosphatase 1 antibody
      • SH2 domain containing inositol phosphatase 1 antibody
      • SH2 domain-containing inositol phosphatase 1 antibody
      • SH2 domain-containing inositol-5''-phosphatase 1 antibody
      • SHIP-1 antibody
      • SHIP1 antibody
      • SHIP1_HUMAN antibody
      • Signaling inositol polyphosphate 5 phosphatase SIP 145 antibody
      • SIP-145 antibody
      • SIP145 antibody
      see all

    Anti-SHIP (phospho Y1020) antibody [EPR425] images

    • All lanes : Anti-SHIP (phospho Y1020) antibody [EPR425] (ab96402) at 1/2000 dilution

      Lane 1 : Raji whole cell lysate - untreated
      Lane 2 : Raji whole cell lysate - treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 133 kDa
      Observed band size : 145 kDa (why is the actual band size different from the predicted?)

      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

    References for Anti-SHIP (phospho Y1020) antibody [EPR425] (ab96402)

    ab96402 has not yet been referenced specifically in any publications.

    Product Wall

    The name we chose for ab96402 was based off of the fact that the original phospho site identified was in rat at Tyrosine 1020, .

    In the human protein, Tyrosine 1022 is the phosphosite, based on teh numbe...

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