Overview

  • Product nameAnti-SHMT1 antibodySee all SHMT1 primary antibodies ...
  • Description
    Mouse monoclonal to SHMT1
  • Tested applicationsWB, IHC-P, Flow Cyt more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 374-483 of Human SHMT1

Properties

Applications

Our Abpromise guarantee covers the use of ab55736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 53 kDa.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 0.1µg for 106 cells.

Target

  • FunctionInterconversion of serine and glycine.
  • PathwayOne-carbon metabolism; tetrahydrofolate interconversion.
  • Sequence similaritiesBelongs to the SHMT family.
  • Cellular localizationCytoplasm.
  • Target information above from: UniProt accession P34896 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CSHMT antibody
    • Cytoplasmic serine hydroxymethyltransferase antibody
    • cytosolic antibody
    • GLYC_HUMAN antibody
    • Glycine hydroxymethyltransferase antibody
    • Serine hydroxymethyltransferase 1 (soluble) antibody
    • Serine hydroxymethyltransferase antibody
    • Serine hydroxymethyltransferase, cytosolic antibody
    • Serine methylase antibody
    • SHMT antibody
    • Shmt1 antibody
    see all

Anti-SHMT1 antibody images

  • Overlay histogram showing HeLa cells stained with ab55736 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55736, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • SHMT1 antibody (ab55736) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human colon tissue.


  • Predicted band size : 53 kDa
    SHMT1 antibody (ab55736) at 1ug/lane + HeLa cell lysate at 25ug/lane.

References for Anti-SHMT1 antibody (ab55736)

ab55736 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Alfredo Castello

Verified customer

Submitted Apr 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"