Overview

  • Product nameAnti-SHMT1 antibody
    See all SHMT1 primary antibodies
  • Description
    Mouse monoclonal to SHMT1
  • Tested applicationsWB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 374-483 of Human SHMT1

  • General notesAb170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab55736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 53 kDa.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 0.1µg for 106 cells.

Target

  • FunctionInterconversion of serine and glycine.
  • PathwayOne-carbon metabolism; tetrahydrofolate interconversion.
  • Sequence similaritiesBelongs to the SHMT family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSHMT antibody
    • Cytoplasmic serine hydroxymethyltransferase antibody
    • cytosolic antibody
    • GLYC_HUMAN antibody
    • Glycine hydroxymethyltransferase antibody
    • Serine hydroxymethyltransferase 1 (soluble) antibody
    • Serine hydroxymethyltransferase antibody
    • Serine hydroxymethyltransferase, cytosolic antibody
    • Serine methylase antibody
    • SHMT antibody
    • Shmt1 antibody
    see all

Anti-SHMT1 antibody images

  • Overlay histogram showing HeLa cells stained with ab55736 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55736, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • SHMT1 antibody (ab55736) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human colon tissue.


  • Predicted band size : 53 kDa
    SHMT1 antibody (ab55736) at 1ug/lane + HeLa cell lysate at 25ug/lane.

References for Anti-SHMT1 antibody (ab55736)

This product has been referenced in:
  • Kim SK  et al. Differential expression of enzymes associated with serine/glycine metabolism in different breast cancer subtypes. PLoS One 9:e101004 (2014). WB ; Human . Read more (PubMed: 24979213) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Alfredo Castello

Verified customer

Submitted Apr 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"