Overview

  • Product name
  • Description
    Rabbit polyclonal to SHMT2
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-483 of Human SHMT2 (P34897).

  • Positive control
    • 293T and HeLa whole cell lysates, HeLa cells, HeLa xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab155230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/5000 - 1/20000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function
    Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA.
  • Pathway
    One-carbon metabolism; tetrahydrofolate interconversion.
  • Sequence similarities
    Belongs to the SHMT family.
  • Cellular localization
    Mitochondrion. Mitochondrion matrix > mitochondrion nucleoid. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis secretory sperm binding protein Li 51e antibody
    • GLY A+ antibody
    • GLYA antibody
    • glycine auxotroph A, human complement for hamster antibody
    • Glycine hydroxymethyltransferase antibody
    • GLYM_HUMAN antibody
    • HEL S 51e antibody
    • mitochondrial antibody
    • Serine hydroxymethyltransferase 2 (mitochondrial) antibody
    • Serine hydroxymethyltransferase antibody
    • Serine hydroxymethyltransferase mitochondrial antibody
    • Serine methylase antibody
    • SHMT 2 antibody
    • SHMT antibody
    • SHMT2 antibody
    see all

Images

  • All lanes : Anti-SHMT2 antibody (ab155230) at 1/10000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 56 kDa
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling SHMT2 with ab155230 at 1/500. Image at the bottom costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded HeLa xenograft labeling SHMT2 with ab155230 antibody at 1/500.

References

ab155230 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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