Overview

  • Product name
  • Description
    Rabbit polyclonal to SHMT2
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    A region within synthetic peptide: IASENFCSRA ALEALGSCLN NKYSEGYPGK RYYGGAEVVD EIELLCQRRA, corresponding to N terminal amino acids 74-123 of Human SHMT2

  • Positive control
    • Human heart. HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab64417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA.
  • Pathway
    One-carbon metabolism; tetrahydrofolate interconversion.
  • Sequence similarities
    Belongs to the SHMT family.
  • Cellular localization
    Mitochondrion. Mitochondrion matrix > mitochondrion nucleoid. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis secretory sperm binding protein Li 51e antibody
    • GLY A+ antibody
    • GLYA antibody
    • glycine auxotroph A, human complement for hamster antibody
    • Glycine hydroxymethyltransferase antibody
    • GLYM_HUMAN antibody
    • HEL S 51e antibody
    • mitochondrial antibody
    • Serine hydroxymethyltransferase 2 (mitochondrial) antibody
    • Serine hydroxymethyltransferase antibody
    • Serine hydroxymethyltransferase mitochondrial antibody
    • Serine methylase antibody
    • SHMT 2 antibody
    • SHMT antibody
    • SHMT2 antibody
    see all

Images

  • Anti-SHMT2 antibody (ab64417) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • ab64417, at 4.0µg/ml, staining myocardial cells in paraffin-embedded human heart tissue.

References

This product has been referenced in:
  • Singh R  et al. Proteomic identification of mitochondrial targets of arginase in human breast cancer. PLoS One 8:e79242 (2013). WB ; Human . Read more (PubMed: 24223914) »
  • Leivonen SK  et al. Identification of miR-193b Targets in Breast Cancer Cells and Systems Biological Analysis of Their Functional Impact. Mol Cell Proteomics 10:M110.005322 (2011). WB ; Human . Read more (PubMed: 21512034) »

See all 2 Publications for this product

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