Anti-SHMT2 antibody (ab88664)

Overview

  • Product nameAnti-SHMT2 antibody
    See all SHMT2 primary antibodies
  • Description
    Mouse polyclonal to SHMT2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human SHMT2 protein (NP_005403.2).

  • Positive control
    • Human spleen tissue lysate. Hela cell lysate. SHMT2 transfected 293T cell lysate. HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionContributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA.
  • PathwayOne-carbon metabolism; tetrahydrofolate interconversion.
  • Sequence similaritiesBelongs to the SHMT family.
  • Cellular localizationMitochondrion. Mitochondrion matrix > mitochondrion nucleoid. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • GLY A+ antibody
    • GLYA antibody
    • glycine auxotroph A, human complement for hamster antibody
    • Glycine hydroxymethyltransferase antibody
    • GLYM_HUMAN antibody
    • mitochondrial antibody
    • Serine hydroxymethyltransferase 2 (mitochondrial) antibody
    • Serine hydroxymethyltransferase antibody
    • Serine hydroxymethyltransferase mitochondrial antibody
    • Serine methylase antibody
    • SHMT 2 antibody
    • SHMT antibody
    • SHMT2 antibody
    see all

Anti-SHMT2 antibody images

  • Anti-SHMT2 antibody (ab88664) at 1 µg/ml + human spleen tissue lysate at 50 µg

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • Anti-SHMT2 antibody (ab88664) at 1 µg/ml + Hela cell lysate at 50 µg

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-SHMT2 antibody (ab88664) at 1 µg/ml

    Lane 1 : SHMT2 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 56 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab88664, at 10 µg/ml, staining SHMT2 in Hela cells by Immunofluorescence.

References for Anti-SHMT2 antibody (ab88664)

ab88664 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat)
Loading amount 20 µg
Specification Jurkat
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Alfredo Castello

Verified customer

Submitted Apr 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"