Overview

  • Product name
  • Description
    Mouse monoclonal to SHOX2
  • Tested applications
    Suitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment: SPELKDRKDD AKGMEDEGQT KIKQRRSRTN FTLEQLNELE RLFDETHYPD AFMREELSQR LGLSEARVQV WFQNRRAKCR KQENQLHK, corresponding to amino acids 117-205 of Human SHOX2

Properties

Applications

Our Abpromise guarantee covers the use of ab55740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-Fr
  • Application notes
    WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May be a growth regulator and have a role in specifying neural systems involved in processing somatosensory information, as well as in face and body structure formation.
    • Tissue specificity
      Expressed in heart, skeletal muscle, liver, lung, bone marrow fibroblast, pancreas and placenta.
    • Sequence similarities
      Belongs to the paired homeobox family. Bicoid subfamily.
      Contains 1 homeobox DNA-binding domain.
    • Developmental stage
      Expressed during cranofacial development as well as in heart.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Homeobox protein Og12X antibody
      • OG 12 antibody
      • OG 12X antibody
      • OG12 antibody
      • OG12X antibody
      • OGI 2X antibody
      • OGI2X antibody
      • Paired related homeobox protein SHOT antibody
      • Paired-related homeobox protein SHOT antibody
      • Short stature homeobox 2 antibody
      • Short stature homeobox homolog antibody
      • Short stature homeobox protein 2 antibody
      • SHOT antibody
      • SHOX 2 antibody
      • SHOX homologous gene on chromosome 3 antibody
      • SHOX2 antibody
      • SHOX2_HUMAN antibody
      see all

    Anti-SHOX2 antibody images

    • ab55740 staining SHOX2 in mouse embryonic spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/500 in PBS + 10% horse serum + 0.1% Triton X-100 in PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview



    • Predicted band size : 35 kDa
      SHOX2 antibody (ab55740) at 1ug/lane + PC-12 cell lysate at 25ug/lane.

    References for Anti-SHOX2 antibody (ab55740)

    ab55740 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Sample
    Mouse Tissue sections (Mouse embryonic spinal cord (cryosections))
    Specification
    Mouse embryonic spinal cord (cryosections)
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Dr. Frederic Clotman

    Verified customer

    Submitted Jan 27 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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