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Rabbit polyclonal to SHOX2
Human, ZebrafishPredicted to work with:
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
Synthetic peptide, corresponding to a region within internal sequence amino acids 179 - 228 (SEARVQVWFQNRRAKCRKQENQLHKGVLIGAASQFEACRVAPYVNVGAL R) of Human SHOX2, (NP_006875)
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.Can be blocked with Human SHOX2 peptide (ab105429). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
May be a growth regulator and have a role in specifying neural systems involved in processing somatosensory information, as well as in face and body structure formation.
Expressed in heart, skeletal muscle, liver, lung, bone marrow fibroblast, pancreas and placenta.
Belongs to the paired homeobox family. Bicoid subfamily.
Contains 1 homeobox DNA-binding domain.
Expressed during cranofacial development as well as in heart.
Information by UniProt
- Homeobox protein Og12X antibody
- OG 12 antibody
- OG 12X antibody
Western blot - SHOX2 antibody (ab82918)
Anti-SHOX2 antibody (ab82918) at 1 µg/ml + 721_B cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
35 kDaObserved band size:
40 kDa (why is the actual band size different from the predicted?
)Additional bands at:
20 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-SHOX2 antibody (ab82918)
All lanes :
Anti-SHOX2 antibody (ab82918) at 1 µg/mlLane 1 :
MarkerLane 2 :
Zebrafish brain homogenate at 20 µgLane 3 :
Zebrafish heart homogenate at 20 µgLane 4 :
Zebrafish liver homogenate at 20 µgSecondaryAll lanes :
Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
35 kDaObserved band size:
41 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
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