• Product nameAnti-SHP1 (phospho Y536) antibody
    See all SHP1 primary antibodies
  • Description
    Rabbit polyclonal to SHP1 (phospho Y536)
  • SpecificityPhospho-Tyr536-SHP1 specific. The antibody detects a 68 kDa band on SDS-PAGE immunoblots of human Jurkat cells treated with pervanadate (not observed in control cells).
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phospho-Tyr536-SHP1 peptide (coupled to carrier protein) corresponding to amino acids around tyrosine 536 in human SHP1. The sequence is highly conserved in rat and mouse SHP1.

  • Positive control
    • This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2



Our Abpromise guarantee covers the use of ab41437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
ELISA 1/2000.
ICC/IF 1/200.


  • FunctionPlays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • Tissue specificityIsoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    Phosphorylated on serine and tyrosine residues.
  • Cellular localizationCytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70Z-SHP antibody
    • EC antibody
    • HCP antibody
    • HCPH antibody
    • Hematopoietic cell phosphatase antibody
    • Hematopoietic cell protein tyrosine phosphatase antibody
    • Hematopoietic cell protein-tyrosine phosphatase antibody
    • HPTP1C antibody
    • Protein tyrosine phosphatase 1C antibody
    • Protein tyrosine phosphatase non receptor type 6 antibody
    • Protein tyrosine phosphatase SHP1 antibody
    • Protein-tyrosine phosphatase 1C antibody
    • protein-tyrosine phosphatase SHP 1 antibody
    • Protein-tyrosine phosphatase SHP-1 antibody
    • PTN6_HUMAN antibody
    • PTP 1C antibody
    • PTP-1C antibody
    • PTP1C antibody
    • Ptpn6 antibody
    • SH PTP 1 antibody
    • SH PTP1 antibody
    • SH-PTP1 antibody
    • SHP 1 antibody
    • SHP 1L antibody
    • SHP1 antibody
    • SHP1L antibody
    • tyrosine protein phosphatase non receptor type 6 antibody
    • Tyrosine-protein phosphatase non-receptor type 6 antibody
    see all

Anti-SHP1 (phospho Y536) antibody images

  • ICC/IF image of ab41437 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab41437 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Lanes 1 - 2 : anti-SHP1 (C-terminal)
    Lanes 3 - 5 : Anti-SHP1 (phospho Y536) antibody (ab41437) at 1/1000 dilution

    Lane 1 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
    Lane 2 : human Jurkat cells treated with 1 mM pervanadate for 30 min, blot exposed to alkaline phosphatase before probing
    Lane 3 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
    Lane 4 : human Jurkat cells treated with 1 mM pervanadate for 30 min, blot exposed to alkaline phosphatase before probing
    Lane 5 : human Jurkat cells treated with 1 mM pervanadate for 30 min. with phospho-Tyr536-SHP1 peptide

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.

References for Anti-SHP1 (phospho Y536) antibody (ab41437)

This product has been referenced in:
  • Zhou X  et al. Leishmania infantum-chagasi activates SHP-1 and reduces NFAT5/TonEBP activity in the mouse kidney inner medulla. Am J Physiol Renal Physiol 307:F516-24 (2014). Read more (PubMed: 24990897) »
  • Kim KA  et al. Calpain-dependent cleavage of SHP-1 and SHP-2 is involved in the dephosphorylation of Jurkat T cells induced by Entamoeba histolytica. Parasite Immunol 32:176-83 (2010). WB ; Human . Read more (PubMed: 20398180) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (T lymphocyte)
Specification T lymphocyte
Treatment pathogen & H2O2
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 25 2014