Anti-SHP1 (phospho Y536) antibody (ab51171)

Overview

  • Product nameAnti-SHP1 (phospho Y536) antibodySee all SHP1 primary antibodies ...
  • Description
    Rabbit polyclonal to SHP1 (phospho Y536)
  • Specificityab51171 detects endogenous levels of SHP1 only when phosphorylated at tyrosine 536.
  • Tested applicationsELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human SHP1 around the phosphorylation site of tyrosine 536 (S-E-YP-G-N).

  • Positive control
    • Human breast carcinoma tissue and RAW264.7 cell extracts treated with EGF (200ng/ml, 5min).

Properties

Applications

Our Abpromise guarantee covers the use of ab51171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P Use at an assay dependent dilution.
WB 1/300 - 1/1000. Predicted molecular weight: 68 kDa.

Target

  • FunctionPlays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
  • Tissue specificityIsoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
    Contains 2 SH2 domains.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications
    Phosphorylated on serine and tyrosine residues.
  • Cellular localizationCytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70 kda SHP 1L protein antibody
    • 70 kda SHP1L protein antibody
    • 70Z-SHP antibody
    • EC 3.1.3.48 antibody
    • HCP antibody
    • HCPH antibody
    • Hematopoietic cell phosphatase antibody
    • Hematopoietic cell protein tyrosine phosphatase antibody
    • Hematopoietic cell protein-tyrosine phosphatase antibody
    • HPTP 1C antibody
    • HPTP1C antibody
    • Protein tyrosine phosphatase 1C antibody
    • Protein tyrosine phosphatase non receptor type 6 antibody
    • Protein tyrosine phosphatase SHP 1 antibody
    • Protein tyrosine phosphatase SHP1 antibody
    • Protein-tyrosine phosphatase 1C antibody
    • protein-tyrosine phosphatase SHP 1 antibody
    • Protein-tyrosine phosphatase SHP-1 antibody
    • PTN6_HUMAN antibody
    • PTP 1C antibody
    • PTP-1C antibody
    • PTP1C antibody
    • PTPN 6 antibody
    • PTPN6 antibody
    • SH PTP 1 antibody
    • SH PTP1 antibody
    • SH-PTP1 antibody
    • SHP 1 antibody
    • SHP 1L antibody
    • SHP1 antibody
    • SHP1L antibody
    • SHPTP 1 antibody
    • SHPTP1 antibody
    • Tyrosine protein phosphatase non receptor type 6 antibody
    • Tyrosine-protein phosphatase non-receptor type 6 antibody
    see all

Anti-SHP1 (phospho Y536) antibody images

  • ab51171 at 1/50 dilution staining SHP1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.


  • Predicted band size : 68 kDa


    Western blot analysis of extracts from RAW264.7 cells treated with EGF (200ng/ml, 5 minutes)

References for Anti-SHP1 (phospho Y536) antibody (ab51171)

This product has been referenced in:
  • Abbonante V  et al. Discoidin domain receptor 1 protein is a novel modulator of megakaryocyte-collagen interactions. J Biol Chem 288:16738-46 (2013). Human . Read more (PubMed: 23530036) »
  • Ketroussi F  et al. Lymphocyte Cell-Cycle Inhibition by HLA-G Is Mediated by Phosphatase SHP-2 and Acts on the mTOR Pathway. PLoS One 6:e22776 (2011). WB ; Human . Read more (PubMed: 21887223) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Jurkat)
Specification Jurkat
Preparation Cell harvesting/tissue preparation method: human Jurkat cells treated with pervanadate (1 mM) for 30 min
Sample buffer: complete RPMI media
Fixation Paraformaldehyde
Permeabilization Yes - methanol
Gating Strategy Jurkat
Username

Abcam user community

Verified customer

Submitted Feb 18 2013

Thank you foryour reply.

The buffers for these product do not contain stabilizing proteins.



Ab51171: Preservative: 0.02% Sodium Azide

Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium ...

Read More

Thank you for your reply.

Unfortunately none of these products has yet been tested in flow cytometry. While we do try and test as many products as we can in a large number of applications, it does seem as this group has not yet been though...

Read More

Thank you for your phone call. As I mentioned, because these are phospho-specific antibodies, we recommend using BSA as a blocking agent instead of milk. If this does not help to improve your results, please let me knowyour original order or PO numbera...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"