Anti-SHP1 (phospho Y536) antibody (ab51171)
- Product nameAnti-SHP1 (phospho Y536) antibodySee all SHP1 primary antibodies ...
- DescriptionRabbit polyclonal to SHP1 (phospho Y536)
- Specificityab51171 detects endogenous levels of SHP1 only when phosphorylated at tyrosine 536.
- Tested applicationsELISA, IHC-P, WB more details
- Species reactivityReacts with: Mouse, Human
Synthetic phosphopeptide derived from human SHP1 around the phosphorylation site of tyrosine 536 (S-E-YP-G-N).
- Positive control
- Human breast carcinoma tissue and RAW264.7 cell extracts treated with EGF (200ng/ml, 5min).
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesab51171 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab51171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||IHC-P: Use at an assay dependent dilution.|
|WB||WB: 1/300 - 1/1000. Predicted molecular weight: 68 kDa.|
- FunctionPlays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
- Tissue specificityIsoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
- Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain.
modificationsPhosphorylated on serine and tyrosine residues.
- Cellular localizationCytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
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Anti-SHP1 (phospho Y536) antibody images
ab51171 at 1/50 dilution staining SHP1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
Predicted band size : 68 kDa
Western blot analysis of extracts from RAW264.7 cells treated with EGF (200ng/ml, 5 minutes)
References for Anti-SHP1 (phospho Y536) antibody (ab51171)
This product has been referenced in:
- Abbonante V et al. Discoidin domain receptor 1 protein is a novel modulator of megakaryocyte-collagen interactions. J Biol Chem 288:16738-46 (2013). Human . Read more (PubMed: 23530036) »
- Ketroussi F et al. Lymphocyte Cell-Cycle Inhibition by HLA-G Is Mediated by Phosphatase SHP-2 and Acts on the mTOR Pathway. PLoS One 6:e22776 (2011). WB ; Human . Read more (PubMed: 21887223) »