Anti-SHP2 antibody (ab9214)
Key features and details
- Goat polyclonal to SHP2
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-SHP2 antibody
See all SHP2 primary antibodies -
Description
Goat polyclonal to SHP2 -
Host species
Goat -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Pig -
Immunogen
Synthetic peptide corresponding to Human SHP2 aa 550 to the C-terminus (C terminal). (NP_001317366.1)
Database link: NP_002825.3 -
Positive control
- WB: Human muscle lysate. ICC/IF: HeLa cells Flow Cyt (intra): A431 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
1 hour primary incubation is recommended for this product. |
|
ICC/IF | (1) |
Use a concentration of 10 µg/ml.
|
Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 10 µg/ml. |
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
Target
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Function
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. -
Tissue specificity
Widely expressed, with highest levels in heart, brain, and skeletal muscle. -
Involvement in disease
Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1) [MIM:151100]. It is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
Defects in PTPN11 are the cause of Noonan syndrome type 1 (NS1) [MIM:163950]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. Some patients with Noonan syndrome type 1 develop multiple giant cell lesions of the jaw or other bony or soft tissues, which are classified as pigmented villomoduolar synovitis (PVNS) when occurring in the jaw or joints. Note=Mutations in PTPN11 account for more than 50% of the cases. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS1 inheritance is autosomal dominant.
Defects in PTPN11 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor.
Defects in PTPN11 are a cause of metachondromatosis (MC) [MIM:156250]. It is a skeletal disorder with radiologic fetarures of both multiple exostoses and Ollier disease, characterized by the presence of multiple enchondromas and osteochondroma-like lesions. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain. -
Domain
The SH2 domains repress phosphatase activity. Binding of these domains to phosphotyrosine-containing proteins relieves this auto-inhibition, possibly by inducing a conformational change in the enzyme. -
Post-translational
modificationsPhosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5781 Human
- Entrez Gene: 25622 Rat
- Omim: 176876 Human
- SwissProt: Q06124 Human
- SwissProt: P41499 Rat
- Unigene: 506852 Human
- Unigene: 98209 Rat
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Alternative names
- BPTP3 antibody
- CFC antibody
- JMML antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% triton-permeabilized HeLa cells staining SHP2 antibody with ab9214 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. DAPI was used as a nuclear counterstain.
Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody at 2ug/ml
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Flow cytometric analysis of paraformaldehyde-fixed, 0.5% Triton-permeabilized A431 cells (blue) staining SHP2 with ab9214 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 1ug/ml. Primary incubation carried out for one hour.
IgG control: Unimmunized goat IgG followed by Alexa Fluor 488 secondary antibody (black).
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Anti-SHP2 antibody (ab9214) at 2 µg/ml + Human muscle lysate at 35 µg
Predicted band size: 68 kDa -
ab9214 staining (2
µ g/ml) of Human Muscle lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab9214 staining (2µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab9214 has been referenced in 5 publications.
- Ding Y et al. Tyrosine phosphatase SHP2 exacerbates psoriasis-like skin inflammation in mice via ERK5-dependent NETosis. MedComm (2020) 3:e120 (2022). PubMed: 35281792
- Sugimoto A et al. SLAM family member 8 is expressed in and enhances the growth of anaplastic large cell lymphoma. Sci Rep 10:2505 (2020). PubMed: 32054954
- Sugimoto A et al. SLAM family member 8 is involved in oncogenic KIT-mediated signalling in human mastocytosis. Exp Dermatol 27:641-646 (2018). PubMed: 29498772
- Liu HP et al. Association of supervillin with KIR2DL1 regulates the inhibitory signaling of natural killer cells. Cell Signal 23:487-96 (2011). WB ; Human . PubMed: 21070852
- Takagi K et al. Modulation of TNF-alpha gene expression by IFN-gamma and pamidronate in murine macrophages: regulation by STAT1-dependent pathways. J Immunol 174:1801-10 (2005). WB ; Mouse . PubMed: 15699106