Anti-SHP2 (phospho S576) antibody (ab17940)

Overview

• Product name
  Anti-SHP2 (phospho S576) antibody
  See all SHP2 primary antibodies
• Description
  Rabbit polyclonal to SHP2 (phospho S576)
• Tested applications
  Suitable for: WBmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human SHP2 that contains serine 576.

• Positive control
  • Hek293 cells treated with PMA.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  Preservative: 0.05% Sodium Azide
  Constituents: 50% Glycerol, Dulbecco's PBS (without Mg2+ and Ca2+), 1mg/ml BSA. pH 7.3
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Purification notes
  Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated SHP2. The final product is generated by affinity chromatography using a SHP2-derived peptide that is phosphorylated at serine 576.
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Phosphatases

  • Neuroscience
  • Sensory System
  • Auditory system

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• Function
  Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.
• Tissue specificity
  Widely expressed, with highest levels in heart, brain, and skeletal muscle.
• Involvement in disease
  Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1) [MIM:151100]. It is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
  Defects in PTPN11 are the cause of Noonan syndrome type 1 (NS1) [MIM:163950]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. Some patients with Noonan syndrome type 1 develop multiple giant cell lesions of the jaw or other bony or soft tissues, which are classified as pigmented villomoduolar synovitis (PVNS) when occurring in the jaw or joints. Note=Mutations in PTPN11 account for more than 50% of the cases. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS1 inheritance is autosomal dominant.
  Defects in PTPN11 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor.
  Defects in PTPN11 are a cause of metachondromatosis (MC) [MIM:156250]. It is a skeletal disorder with radiologic fetarures of both multiple exostoses and Ollier disease, characterized by the presence of multiple enchondromas and osteochondroma-like lesions.
• Sequence similarities
  Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
  Contains 2 SH2 domains.
  Contains 1 tyrosine-protein phosphatase domain.
• Domain
  The SH2 domains repress phosphatase activity. Binding of these domains to phosphotyrosine-containing proteins relieves this auto-inhibition, possibly by inducing a conformational change in the enzyme.
• Post-translational
  modifications
  Phosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins.
• Cellular localization
  Cytoplasm.

• Target information above from: UniProt accession Q06124 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 5781 Human
  • Entrez Gene: 19247 Mouse
  • Entrez Gene: 25622 Rat
  • Omim: 176876 Human
  • SwissProt: Q06124 Human
  • SwissProt: P35235 Mouse
  • SwissProt: P41499 Rat
  • Unigene: 506852 Human

  • Unigene: 474046 Mouse
  • Unigene: 8681 Mouse
  • Unigene: 98209 Rat

  see all
• Alternative names
  • BPTP3 antibody
  • CFC antibody
  • JMML antibody

  • METCDS antibody
  • MGC14433 antibody
  • NS1 antibody
  • OTTHUMP00000166107 antibody
  • OTTHUMP00000166108 antibody
  • Protein tyrosine phosphatase 2 antibody
  • Protein tyrosine phosphatase 2C antibody
  • Protein tyrosine phosphatase non receptor type 11 antibody
  • Protein-tyrosine phosphatase 1D antibody
  • Protein-tyrosine phosphatase 2C antibody
  • PTN11_HUMAN antibody
  • PTP-1D antibody
  • PTP-2C antibody
  • PTP1D antibody
  • PTP2C antibody
  • PTPN11 antibody
  • SAP2 antibody
  • SH-PTP2 antibody
  • SH-PTP3 antibody
  • SH2 domain containing protein tyrosine phosphatase 2 antibody
  • SHP 2 antibody
  • SHP-2 antibody
  • Shp2 antibody
  • SHPTP2 antibody
  • SHPTP3 antibody
  • Syp antibody
  • Tyrosine-protein phosphatase non-receptor type 11 antibody

  see all

Protocols

• Recommended WB protocol with ab17940