Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab80129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 193 kDa (predicted molecular weight: 193 kDa).

Target

  • FunctionE3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.
  • Tissue specificityBroadly expressed.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 PHD-type zinc finger.
    Contains 1 RING-type zinc finger.
  • DomainThe RING finger mediates E3 ubiquitin ligase activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA545I5.2 antibody
    • E3 ubiquitin-protein ligase SHPRH antibody
    • FLJ27258 antibody
    • FLJ37625 antibody
    • FLJ45012 antibody
    • FLJ90837 antibody
    • histone-linker antibody
    • KIAA2023 antibody
    • MGC134886 antibody
    • PHD and RING finger domain-containing helicase antibody
    • Shprh antibody
    • SHPRH_HUMAN antibody
    • SNF2 antibody
    • SNF2 histone linker PHD RING helicase antibody
    see all

Anti-SHPRH antibody images

  • Anti-SHPRH antibody (ab80129) at 1/1000 dilution + HEK293 whole cell lysate at 50 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 193 kDa
    Observed band size : 175 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ICC/IF image of ab80129 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80129, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) SHSY5Y cells at 5µg/ml.
  • Anti-SHPRH antibody (ab80129) at 1 µg/ml + SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 193 kDa
    Observed band size : 104,193 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 172 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes
    This antibody was raised against an immunogen that is predicted to recognize isoforms (1,2,3,4 and 5) of E3 ubiquitin-protein ligase SHPRH. The predicted molecular weights of isoforms (1,2,3,4 and 5) are 193 kDa, 104kDa, 17 kDa, 190 kDa and 122 kDa respectively.

References for Anti-SHPRH antibody (ab80129)

ab80129 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HCT116 cell lines)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 30 µg
Specification HCT116 cell lines
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Jul 14 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"