Overview

  • Product nameAnti-SHROOM4 antibody
  • Description
    Rabbit polyclonal to SHROOM4
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 1170-1300 of Human SHROOM4.

  • Positive control
    • Human cerebral cortex tissue; U 251 MG cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab121316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.

Target

  • FunctionProbable regulator of cytoskeletal architecture that plays an important role in development. May regulate cellular and cytoskeletal architecture by modulating the spatial distribution of myosin II.
  • Tissue specificityExpressed in all fetal and adult tissues investigated. Expressed in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. In brain regions detected in cerebellum, cerebral cortex, medulla, spinal cord, occipital pole, frontal lobe, temporal lobe and putamen. The expression is strongest in the medulla and weakest in the cerebral cortex.
  • Involvement in diseaseDefects in SHROOM4 are the cause of mental retardation syndromic X-linked Stocco dos Santos type (SDSX) [MIM:300434]. A syndrome characterized by severe mental retardation with hyperactivity, aggressive behavior, delayed or no speech, and seizures. Additional features include congenital bilateral hip luxation, short stature, and kyphosis.
    Note=A chromosomal aberration involving SHROOM4 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with FBXO25.
    Note=A chromosomal aberration involving SHROOM4 is a cause of X-linked mental retardation (XLMR). Translocation t(X;19).
  • Sequence similaritiesBelongs to the Shroom family.
    Contains 1 ASD2 domain.
    Contains 1 PDZ (DHR) domain.
  • Cellular localizationCytoplasm > cytoskeleton. Shows partial colocalization with the cytoplasmic pool of F-actin.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein Shroom4 antibody
    • RP11-119E20.1 antibody
    • Second homolog of apical protein antibody
    • SHAP antibody
    • SHRM4_HUMAN antibody
    • Shroom family member 4 antibody
    • SHROOM4 antibody
    see all

Anti-SHROOM4 antibody images

  • ab121316, at 1/50 dilution, staining SHROOM4 in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
  • ab1216316, at 4µg/ml, staining SHROOM4 in in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).

References for Anti-SHROOM4 antibody (ab121316)

ab121316 has not yet been referenced specifically in any publications.

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