Anti-SBDS antibody (ab154222)
Key features and details
- Rabbit polyclonal to SBDS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-SBDS antibody
See all SBDS primary antibodies -
Description
Rabbit polyclonal to SBDS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis -
Immunogen
Recombinant fragment corresponding to Human SBDS aa 1-250.
Database link: Q9Y3A5 -
Positive control
- Recombinant Human SBDS protein (ab99957) can be used as a positive control in WB. 293T, A431, H1299, HepG2, Molt-4, Raji and Mouse liver lysate, HeLa cells, H1299 xenograft tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 29 kDa.
|
|
IHC-P |
1/100 - 1/1000.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 29 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
-
Function
Required for the assembly of mature ribosomes and ribosome biogenesis. Together with EFTUD1, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Required for normal levels of protein synthesis. May play a role in cellular stress resistance. May play a role in cellular response to DNA damage. May play a role in cell proliferation. -
Tissue specificity
Widely expressed. -
Involvement in disease
Defects in SBDS are the cause of Shwachman-Diamond syndrome (SDS) [MIM:260400]. SDS is an autosomal recessive disorder characterized by pancreatic exocrine insufficiency, hematologic dysfunction, and skeletal abnormalities. -
Sequence similarities
Belongs to the SDO1/SBDS family. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Primarily detected in the cytoplasm, and at low levels in nucleus and nucleolus (PubMed:19602484 and PubMed:17475909). Detected in the nucleolus during G1 and G2 phase of the cell cycle, and diffusely distributed in the nucleus during S phase. Detected at the mitotic spindle. Colocalizes with the microtubule organizing center during interphase. - Information by UniProt
-
Database links
- Entrez Gene: 417477 Chicken
- Entrez Gene: 513237 Cow
- Entrez Gene: 51119 Human
- Entrez Gene: 66711 Mouse
- Entrez Gene: 288615 Rat
- Entrez Gene: 100049749 Xenopus laevis
- Entrez Gene: 448365 Xenopus tropicalis
- Omim: 607444 Human
see all -
Alternative names
- 4733401P19Rik antibody
- AI836084 antibody
- CGI 97 antibody
see all
Images
-
All lanes : Anti-SBDS antibody (ab154222) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
Anti-SBDS antibody (ab154222) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells, labelling SBDS with ab154222 at 1/500 dilution (top image), and costained with Hoescht 33342 (bottom image)
-
Immunohistochemical analysis of paraffin embedded Human H1299 xenograft tissue labelling SBDS with ab154222 at a 1/500 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab154222 has not yet been referenced specifically in any publications.