Overview

  • Product name
    Anti-SIAE antibody
  • Description
    Mouse polyclonal to SIAE
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human SIAE protein (NP_733746.1)

  • Positive control
    • Human colon tissue lysate. SIAE transfected 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

Target

  • Relevance
    Sialic acids are acidic 9-carbon sugars typically found at the non-reducing end of sugar chains. They are frequently modified by 9-O-acetylation and this modification is removed by sialic acid acetylesterases. SIAE catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid. SIAE appears to encode both lysosomal and cytosolic sialic acid acetylesterase isoforms (LSE and CSE, respectively). It is widely expressed with high expression in the testis, prostate, and colon. There are three named isoforms.
  • Cellular localization
    Lysosome
  • Database links
  • Alternative names
    • CSE C antibody
    • CSEC antibody
    • Cytosolic sialic acid 9 O acetylesterase homolog antibody
    • H Lse antibody
    • HLse antibody
    • LSE antibody
    • MGC87009 antibody
    • Sialate O acetylesterase antibody
    • Sialic acid acetylesterase antibody
    • Sialic acid specific 9 O acetylesterase antibody
    • Sialic acid specific acetylesterase II antibody
    • YSG2 antibody
    • Ysg2 homolog antibody
    see all

Images

  • Anti-SIAE antibody (ab69300) at 1/500 dilution + human colon tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 21 kDa,26 kDa,32 kDa,35 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-SIAE antibody (ab69300) at 1/500 dilution

    Lane 1 : SIAE transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa

References

ab69300 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab69300.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up