Anti-SIAH Interacting Protein antibody [2103C3a] (ab51288)

Overview

  • Product nameAnti-SIAH Interacting Protein antibody [2103C3a]
    See all SIAH Interacting Protein primary antibodies
  • Description
    Mouse monoclonal [2103C3a] to SIAH Interacting Protein
  • Tested applicationsSuitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human)

  • Positive control
    • HeLa or NIH3T3 whole cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number2103C3a
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 26 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • FunctionMay be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
  • Sequence similaritiesContains 1 CS domain.
    Contains 1 SGS domain.
  • Post-translational
    modifications
    Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations.
  • Cellular localizationNucleus. Cytoplasm. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • CacyBP antibody
    • Calcyclin binding protein antibody
    • Calcyclin-binding protein antibody
    • CYBP_HUMAN antibody
    • GIG 5 antibody
    • GIG5 antibody
    • Growth inhibiting gene 5 antibody
    • Growth inhibiting gene 5 protein antibody
    • hCacyBP antibody
    • MGC87971 antibody
    • PNAS 107 antibody
    • PNAS107 antibody
    • RP1 102G20.6 antibody
    • S100A6 binding protein antibody
    • S100A6-binding protein antibody
    • S100A6BP antibody
    • Siah-interacting protein antibody
    • SIP antibody
    see all

Anti-SIAH Interacting Protein antibody [2103C3a] images

References for Anti-SIAH Interacting Protein antibody [2103C3a] (ab51288)

This product has been referenced in:
  • Czeredys M  et al. Expression of genes encoding the calcium signalosome in cellular and transgenic models of Huntington's disease. Front Mol Neurosci 6:42 (2013). WB ; Rat . Read more (PubMed: 24324398) »
  • Steiniger SC  et al. Quantitative mass spectrometry identifies drug targets in cancer stem cell-containing side population. Stem Cells 26:3037-46 (2008). WB ; Human . Read more (PubMed: 18802034) »

See all 2 Publications for this product

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Application Western blot
Sample Mouse Cell lysate - other (MCF7 breast cancer)
Loading amount 10 µg
Specification MCF7 breast cancer
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Dr. judith coppinger

Verified customer

Submitted Dec 03 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"