SIAH1 antibody (ab2237)

Product Name 

SIAH1 antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to SIAH1

Immunogen 

Synthetic peptide: SRQTATALPTGTSKC, corresponding to N terminal amino acids 2-16 of Human SIAH1.
BLAST 'SRQTATALPTGTSKC' with or

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Rat
(Due to sequence homology)

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects bands of approximately 23 and 30kDa observed in Human Liver lysates (calculated MW of 31.1kDa and 34.6kDa according to NP_003022.3 and NP_001006611.1).kDa.

Can be blocked with SIAH1 peptide (2-16).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human liver lysate.

Cellular localization 

Predominantly cytoplasmic, but also nuclear

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway

Relevance 

This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: Tris, 0.5% BSA. pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for SIAH1 antibody images, references, products related to ab2237 and other tools.

SIAH1 antibody images:

References for SIAH1 antibody (ab2237)

  This product has been used in: (two most recent references)

Guigon CJ et al. Regulation of beta-catenin by a novel nongenomic action of thyroid hormone beta receptor. Mol Cell Biol 28:4598-608 (2008). WB; Mouse. PubMed: 18474620

Groenman F et al. Hypoxia-inducible factors in the first trimester human lung. J Histochem Cytochem 55:355-63 (2007). PubMed: 17189520

See all 3 publication references for this product.


If you publish research using ab2237 please let us know so that we can cite the reference on this datasheet.

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