Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurology process >> Notch Pathway
Overview
Product name
Anti-SIAH1 antibody
See all SIAH1 products (6) ...
Description
Goat polyclonal to SIAH1
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow, Dog
Immunogen
Synthetic peptide: SRQTATALPTGTSKC, corresponding to N terminal amino acids 2-16 of Human SIAH1.
SRQTATALPT GTSKC
Positive control
Human liver lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway
-
Western blot - SIAH1 antibody (ab2237)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 0.3 µg/ml.Detects a band of approximately 28, 30 kDa (predicted molecular weight: 31, 35 kDa).
IP: Use at an assay dependent concentration.
Target
Relevance
This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
Cellular localization
Predominantly cytoplasmic, but also nuclear
Alternative names
- hSIAH1 antibody
- HUMSIAH antibody
- Seven in absentia homolog 1 (Drosophila) antibody
see all
Database links
- Entrez Gene: 6477 Human
- Entrez Gene: 20437 Mouse
- Entrez Gene: 140941 Rat
- Omim: 602212 Human
- SwissProt: Q8IUQ4 Human
- SwissProt: P61092 Mouse
- SwissProt: Q920M9 Rat
- Unigene: 706828 Human
Anti-SIAH1 antibody images:
Western blot - SIAH1 antibody (ab2237)
Anti-SIAH1 antibody (ab2237) at 0.1 µg/ml + Human liver lysate (RIPA buffer, 30µg total protein per lane)
Predicted band size : 31, 35 kDa
Observed band size : 28,30 kDa (why is the actual band size different from the predicted?)
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
References for Anti-SIAH1 antibody (ab2237)
This product has been referenced in:
- Snider NTet al. Energy determinants GAPDH and NDPK act as genetic modifiers for hepatocyte inclusion formation. J Cell Biol 195:217-29 (2011).Read more (PubMed: 22006949) »
- Fujita Ket al. Positive feedback between p53 and TRF2 during telomere-damage signalling and cellular senescence. Nat Cell Biol 12:1205-12 (2010). WB; Human.Read more (PubMed: 21057505) »
See all 5 publications for this product
Publishing research using ab2237? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"