Overview

  • Product name
    Sialic Acid (NANA) Assay Kit
  • Sample type
    Cell culture supernatant, Urine, Cell culture media
  • Assay type
    Quantitative
  • Sensitivity
    1 µM
  • Range
    0.1 µM - 10 µM
  • Assay time
    0h 40m
  • Product overview

    Sialic Acid (NANA) Assay Kit (ab83375) is a simple and convenient kit for measuring free sialic acid (mainly N-acetylneuraminic acid (NANA)) in a variety of biological samples. The detection is based in an enzyme coupled reaction in which oxidation of free sialic acid creates an intermediate that reacts stoichiometrically with the probe to generate a product that can detected by absorbance (OD = 570 nm) or fluorescence (Ex/Em=535/587 nm).

    The kit measures sialic acid in the linear range of 0.1 to 10 nmol with a detection sensitivity ~1 µM concentration.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone. It is also the name for the most common member of this group, N-acetylneuraminic acid (NANA). Sialic acids are found widely distributed in animal tissues and to a lesser extent in other species ranging from plants and fungi to yeasts and bacteria, mostly in glycoproteins and gangliosides. It has been shown recently that sialic acid level may be associated with developmental and pathological stages.

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Sialic Acid Assay Buffer WM 1 x 25ml
    Sialic Acid Converting Enzyme (lyophilized) Purple 1 vial
    Sialic Acid Development Mix (lyophilized) Green 1 vial
    Sialic Acid Probe (in DMSO) Red 1 x 200µl
    Sialic Acid Standard (10 umol) (lyophilized) Yellow 1 vial
  • Research areas
  • Relevance
    Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone. It is also the name for the most common member of this group, N-acetylneuraminic acid (NANA). Sialic acids are found widely distributed in animal tissues and to a lesser extent in other species ranging from plants and fungi to yeasts and bacteria, mostly in glycoproteins and gangliosides. It has been show recently that sialic acid level may associate with developmental and pathological stages.
  • Alternative names
    • N acetylneuraminic acid
    • NANA

Applications

Our Abpromise guarantee covers the use of ab83375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Images

  • Functional studies - Sialic Acid 5NANA) Assay Kit (ab83375)
    Functional studies - Sialic Acid 5NANA) Assay Kit (ab83375)

    Standard curve (fluorimetric) : mean of duplicates (+/-SD) with background readings subtrated

  • Functional Studies - Sialic Acid (NANA) Assay Kit (ab83375)
    Functional Studies - Sialic Acid (NANA) Assay Kit (ab83375)
    Examples of colorimetric and fluorometric standard Sialic acid curves using ab83375.

Protocols

References

This product has been referenced in:
  • Chen WA  et al. Addition of aGal HyperAcute™ technology to recombinant avian influenza vaccines induces strong low-dose antibody responses. PLoS One 12:e0182683 (2017). Read more (PubMed: 28787006) »
  • Mudd AT  et al. Porcine Milk Oligosaccharides and Sialic Acid Concentrations Vary Throughout Lactation. Front Nutr 3:39 (2016). Functional Studies . Read more (PubMed: 27660754) »

See all 3 Publications for this product

Publishing research using ab83375? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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12 reviews or Q&A

10
Votes: Highest First

I am looking at buying Anti-Lewis antibodies from Abcam, and am in need of standards. The kit ab83375 has these standards, but it is not an ELISA kit, and is therefore not suitable for use by us.



Is it possible for Abcam to please s...

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I am pleased to let you know that the sialic acid standards are now available separately under the product number ab170150.

Has your Sialic Acid (NANA) Assay Kit (ab83375) been demonstrated to work with crude (whole-cell) plant extract? This may be unlikely, since only a few groups have successfully expressed this pathway in plants.

If we do use crude plant extrac...

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To our knowledge only mammalian samples have been tested with this kit and we are not sure whether crude plant extracts would be compatible. The kit is designed to detect free sialic acid.

Re: Sialic Acid (NANA) Assay Kit (ab83375) We are trying to produce NANA in plants by genetic introduction of the mammalian pathway. Some reports indicate that similarly engineered plants can produce up to 5 uM NANA in plant tissue. Your kit looks to h...

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We have used this kit only with mammalian samples, therefore we cannot comment on how well it would work with plant samples. Since the principle is the same, this kit should work with samples from multiple sources including plants. However, you ma...

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Dear Josephine,
Our customer knows molecular weight of sialic acid is 300Da.
So she concerns they will get out when she used 3,000 kDa MWCO UF filter.
Please check again.

Thank you for contacting us.

Yes, the SA will get out into the filtrate. That is the whole purpose of filtering this. The SA will get out into the filtrate and the higher MW interfering agents will be stuck at the top of the filter.
Read More

Dear technical team,

Our customer purchased ab83375 to measure free and hydrolyzed bound form of sialic acid.
But there is no hydrolysis protocol in the manual(page 7). So she would like to receive protocol.
Please let me know if y...

Read More

Thank you for contacting us.

You could try the following protocol for gycoprotein, glycolipid or glycan samples:

Sample is dissolved in a final concentration of 2 M HOAc and heated to 80°C for 3 hours to release sialic acids. Th...

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Hello,

I have a question about your Sialic Acid (NANA) Assay kit (ab83375). I would like to identify bound sialic acid in and on a protozoan oocyst wall surface. In the instructions, I noted that in the instructions this kit will measure fre...

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Thank you very much for your inquiry about the ab83375.

This is a very good question.

I am sorry however that this kit will not be suitable for your needs. Indeed, as you mention, for measuring the bound sialic acid, the sample must...

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Dear Sci Sup, Thank you for your email.   Our customer has more inquiries. She disoolved her sample in final concentration of 100mM HCL and heated 80C for 1hr then applied them her test but, nothing detected.   1. So, she would like to know if ...

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Thank you for contacting us. Could you please describe more fully the problems your customer is encountering? Does she observe the expected results when using the Sialic acid standard provided with the kit? In the protocol provided with the kit the...

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Dear Technical team,   Our customer purchased ab83375 and has inquiry about this kit.   1. Her sample is cell culture supernatant. Can it be used without any preprocessing?    2. This kit detects both free and binded sialic acid? She need t...

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Thank your for your interest in this product. In answer to your questions: 1. Her sample is cell culture supernatant. Can it be used without any preprocessing? Cell culture supernatant should not require pre-processing in order to utilise the k...

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P {margin-top:2px;margin-bottom:2px;} Dear technical team,   Our cusotmer would like to purchase ab83375 and needs to check the applicable sample.   http://www.abcam.com/Sialic-Acid-NANA-Assay-Kit-ab83375.html   I have checked 'Scientific Support' but ...

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Thank you for your inquiry. I can confirm that the sample types tested for this kit are cell and tissue culture supernatants, urine, plasma & serum, and other biological fluids. This does mean media, and not lysate. I f your customer is plan...

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Dear Tech help,  I have a customer who has purchased AB83375 but would like further clarification on the protocol: Regarding how much sample to add to each well, I don't have much sample to "play with" to use to test for best response.  The amou...

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Thank you for your enquiry. I can confirm that the sample volume to be used with ab83375 Sialic Acid (NANA) Assay Kit will very much depend on the levels of this metabolite in the sample. It generally has to be in the linear range of the assay ...

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1-10 of 12 Abreviews or Q&A

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