Anti-Sialoadhesin/CD169 antibody [3D6.112] (ab53443)
Key features and details
- Rat monoclonal [3D6.112] to Sialoadhesin/CD169
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-Sialoadhesin/CD169 antibody [3D6.112]
See all Sialoadhesin/CD169 primary antibodies -
Description
Rat monoclonal [3D6.112] to Sialoadhesin/CD169 -
Host species
Rat -
Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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General notes
Fusion partners: Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
Primary antibody notes
Fusion partners: Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line. -
Clonality
Monoclonal -
Clone number
3D6.112 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
1/50 - 1/100.
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Notes |
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IHC-Fr
1/50 - 1/100. |
Target
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Function
Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. -
Tissue specificity
Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis. -
Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 20612 Mouse
- SwissProt: Q62230 Mouse
- Unigene: 1374 Mouse
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Alternative names
- CD169 antibody
- CD169 antigen antibody
- dJ1009E24.1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab53443 has been referenced in 6 publications.
- Chen Q et al. Resident macrophages restrain pathological adipose tissue remodeling and protect vascular integrity in obese mice. EMBO Rep 22:e52835 (2021). PubMed: 34196465
- Zheng J et al. Evaluation of metastatic niches in distant organs after surgical removal of tumor-bearing lymph nodes. BMC Cancer 18:608 (2018). PubMed: 29848296
- Ibrahim MK et al. Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection. Infect Immun 82:3098-112 (2014). PubMed: 24818662
- Ibrahim MK et al. The malnutrition-related increase in early visceralization of Leishmania donovani is associated with a reduced number of lymph node phagocytes and altered conduit system flow. PLoS Negl Trop Dis 7:e2329 (2013). Mouse . PubMed: 23967356
- Xiong YS et al. Contribution of monocytes Siglec-1 in stimulating T cells proliferation and activation in atherosclerosis. Atherosclerosis 224:58-65 (2012). PubMed: 22789514
- Zollo M et al. Targeting monocyte chemotactic protein-1 synthesis with bindarit induces tumor regression in prostate and breast cancer animal models. Clin Exp Metastasis 29:585-601 (2012). PubMed: 22484917