Overview

  • Product nameAnti-Sialoadhesin antibody [3D6.112]
    See all Sialoadhesin primary antibodies
  • Description
    Rat monoclonal [3D6.112] to Sialoadhesin
  • Tested applicationsSuitable for: IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Purified full length Sialoadhesin protein (Mouse).

  • General notes


    Fusion partners: Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Primary antibody notesFusion partners: Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.
  • ClonalityMonoclonal
  • Clone number3D6.112
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50 - 1/100.
Flow Cyt 1/100 - 1/1000. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionActs as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.
  • Tissue specificityExpressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD169 antibody
    • CD169 antigen antibody
    • dJ1009E24.1 antibody
    • DKFZp667F058 antibody
    • FLJ00051 antibody
    • FLJ00055 antibody
    • FLJ00073 antibody
    • FLJ00411 antibody
    • FLJ32150 antibody
    • Sialic acid binding Ig like lectin 1 antibody
    • sialic acid binding Ig-like lectin 1, sialoadhesin antibody
    • Sialic acid binding immunoglobulin like lectin 1 antibody
    • Sialic acid-binding Ig-like lectin 1 antibody
    • Sialoadhesin antibody
    • Siglec 1 antibody
    • Siglec-1 antibody
    • Siglec1 antibody
    • SN antibody
    • SN_HUMAN antibody
    see all

Anti-Sialoadhesin antibody [3D6.112] images

  • ab53443 staining Sialoadhesin in mouse spleen by Immunohistochemistry (Frozen sections).

References for Anti-Sialoadhesin antibody [3D6.112] (ab53443)

This product has been referenced in:
  • Ibrahim MK  et al. Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection. Infect Immun 82:3098-112 (2014). Read more (PubMed: 24818662) »
  • Ibrahim MK  et al. The malnutrition-related increase in early visceralization of Leishmania donovani is associated with a reduced number of lymph node phagocytes and altered conduit system flow. PLoS Negl Trop Dis 7:e2329 (2013). Mouse . Read more (PubMed: 23967356) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

Unfortunately, we only have the non-conjugated antibody or with a FITC conjugate.

However, you could conjugate the antibody yourself with one of our biotin conjugation kits. Please see the following dat...

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Thank you for your inquiry. The immunogen for this antibody was the full length protein from mouse. The epitope has not been mapped by us, or anyone else to our knowledge. Therefore I am unable to answer your question. We do not know where this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"