Anti-Sialoadhesin antibody (ab94715)

Overview

  • Product nameAnti-Sialoadhesin antibody
    See all Sialoadhesin primary antibodies
  • Description
    Rabbit polyclonal to Sialoadhesin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Dog, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Sialoadhesin.

  • Positive control
    • This antibody gave a positive signal in Human Spleen Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab94715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 183 kDa).

Target

  • FunctionActs as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.
  • Tissue specificityExpressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD169 antibody
    • CD169 antigen antibody
    • dJ1009E24.1 antibody
    • DKFZp667F058 antibody
    • FLJ00051 antibody
    • FLJ00055 antibody
    • FLJ00073 antibody
    • FLJ00411 antibody
    • FLJ32150 antibody
    • Sialic acid binding Ig like lectin 1 antibody
    • sialic acid binding Ig-like lectin 1, sialoadhesin antibody
    • Sialic acid binding immunoglobulin like lectin 1 antibody
    • Sialic acid-binding Ig-like lectin 1 antibody
    • Sialoadhesin antibody
    • Siglec 1 antibody
    • Siglec-1 antibody
    • Siglec1 antibody
    • SN antibody
    • SN_HUMAN antibody
    see all

Anti-Sialoadhesin antibody images

  • Anti-Sialoadhesin antibody (ab94715) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 183 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,50 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes

References for Anti-Sialoadhesin antibody (ab94715)

ab94715 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Pig Cell lysate - other (Alveolar macrophages)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 20000 cells
Specification Alveolar macrophages
Blocking step PBS/0.1% Tween 20/5% skimmed milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

David Winder

Verified customer

Submitted Aug 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"