Anti-Sialoadhesin antibody [HSn 7D2] (ab18619)

Overview

  • Product nameAnti-Sialoadhesin antibody [HSn 7D2]
    See all Sialoadhesin primary antibodies
  • Description
    Mouse monoclonal [HSn 7D2] to Sialoadhesin
  • Tested applicationsSuitable for: ICC/IF, WB, RIA, Flow Cyt, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length hSn(d1-4)Fc protein (purified) (Human).

  • EpitopeWithin domains 1-4.

Properties

Applications

Our Abpromise guarantee covers the use of ab18619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20696778
WB 1/250. 1/250 (see Abreview).
RIA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Fix with acetone.

Target

  • FunctionActs as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.
  • Tissue specificityExpressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD169 antibody
    • CD169 antigen antibody
    • dJ1009E24.1 antibody
    • DKFZp667F058 antibody
    • FLJ00051 antibody
    • FLJ00055 antibody
    • FLJ00073 antibody
    • FLJ00411 antibody
    • FLJ32150 antibody
    • Sialic acid binding Ig like lectin 1 antibody
    • sialic acid binding Ig-like lectin 1, sialoadhesin antibody
    • Sialic acid binding immunoglobulin like lectin 1 antibody
    • Sialic acid-binding Ig-like lectin 1 antibody
    • Sialoadhesin antibody
    • Siglec 1 antibody
    • Siglec-1 antibody
    • Siglec1 antibody
    • SN antibody
    • SN_HUMAN antibody
    see all

Anti-Sialoadhesin antibody [HSn 7D2] images

  • ab18619 staining human peripheral blood mononuclear cells by flow cytometry. The cells were prepared in FACS buffer (PBS, 0.5% FCS, 0.5M EDTA) and fixed in PFA without undergoing permeabilization. Cells were gated on lymphocyte and monocyte populations and incubated with primary antibody (1/25) for 20 minutes at 4°C. The secondary antibody used was Phycoerythrin-conjugated rat polyclonal to mouse Ig, diluted 1/100. The PBMC cells were almost exclusively CD14+ monocytic cells.

    See Abreview

  • ICC/IF image of ab18619 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18619, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Sialoadhesin antibody [HSn 7D2] (ab18619)

This product has been referenced in:
  • Fujita E  et al. Statin attenuates experimental anti-glomerular basement membrane glomerulonephritis together with the augmentation of alternatively activated macrophages. Am J Pathol 177:1143-54 (2010). ICC/IF ; Human . Read more (PubMed: 20696778) »
  • Hartnell A  et al. Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations. Blood 97:288-96 (2001). Read more (PubMed: 11133773) »

See all 3 Publications for this product

Product Wall

One reference listed in the 'Specific references' section does relate use of ab18619 in IHC. This is the Hartnell et al paper. They do not describe the use of antigen retrieval.

I would suggest trying no antigen retrieval along with a citrate...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Monocytes and Macrophages)
Loading amount 100000 cells
Specification Monocytes and Macrophages
Treatment Untreated monocytes or monocytes treated for 5 days with PMA plus 1 day PMA and hydrocortisone to differentiate them to macrophages
Gel Running Conditions Reduced Denaturing (Pierce Precise 4 - 20 % gradient protein gels)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Sep 23 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Peripheral blood mononuclear cells)
Specification Peripheral blood mononuclear cells
Preparation Cell harvesting/tissue preparation method: Cells harvested from whole blood and PBMC isolated through a Ficoll gradient
Sample buffer: FACS buffer (PBS 0.5% FCS 0.5M EDTA)
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy Lympocyte and Monocyte populations
Username

Abcam user community

Verified customer

Submitted Sep 02 2008

I can confirm that ab18619 is supplied in PBS buffer only (sterile and filtered). Please let me know if you need more information about this product,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"