Anti-Sialyl Tn antibody [STn 219] (ab115957)

Overview

  • Product nameAnti-Sialyl Tn antibody [STn 219]
    See all Sialyl Tn primary antibodies
  • Description
    Mouse monoclonal [STn 219] to Sialyl Tn
  • SpecificityThis antibody stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane or luminal staining patterns. It is negative with majority of adult tissue sections.
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Sheep, Human
  • Immunogen

    Ovine submaxillary mucin (OSM).

  • EpitopeNeuAc a GalNacOSer/Thr.
  • Positive control
    • Human gastrointestinal tumor, prostate and ovary carcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab115957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100.
IHC-Fr 1/50 - 1/100.
ICC Use at an assay dependent concentration.

Target

  • RelevanceSialyl-Tn is a carbohydrate antigen overexpressed in several epithelial cancers including breast cancer, and usually associated with poor prognosis. Sialyl-Tn is synthesized by a CMP-Neu5Ac: GalNAc alpha2,6-sialyltransferase: ST6GalNAc I, which catalyzes the transfer of a sialic acid residue in alpha2,6-linkage to the GalNAcalpha1-O-Ser/Thr structure. The resulting disaccharide (Neu5Acalpha2-6GalNAcalpha1-O-Ser/Thr) cannot be further elongated and sialyl-Tn expression results therefore in a shortening of the O-glycan chains
  • Alternative names
    • CD175s antibody
    • Sialosyl Tn antibody
    • sTn antibody

Anti-Sialyl Tn antibody [STn 219] images

  • Overlay histogram showing MCF7 cells stained with ab115957 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115957, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Sialyl Tn antibody [STn 219] (ab115957)

ab115957 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab115957.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"