Anti-Sialyl Tn antibody [STn 219] (FITC) (ab76756)

Overview

  • Product nameAnti-Sialyl Tn antibody [STn 219] (FITC)
    See all Sialyl Tn primary antibodies
  • Description
    Mouse monoclonal [STn 219] to Sialyl Tn (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab76756 stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane or luminal staining patterns. It is negative with majority of adult tissue sections.
  • Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Sheep, Human
  • Immunogen

    Ovine submaxillary mucin (OSM)

  • EpitopeNeuAc alpha GalNacOSer/Thr.
  • Positive control
    • Human gastrointestinal tumor, prostate and ovary carcinomas.

Properties

Applications

Our Abpromise guarantee covers the use of ab76756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2.5µl for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 - 20 µg/ml.

Target

  • RelevanceSialyl-Tn is a carbohydrate antigen overexpressed in several epithelial cancers including breast cancer, and usually associated with poor prognosis. Sialyl-Tn is synthesized by a CMP-Neu5Ac: GalNAc alpha2,6-sialyltransferase: ST6GalNAc I, which catalyzes the transfer of a sialic acid residue in alpha2,6-linkage to the GalNAcalpha1-O-Ser/Thr structure. The resulting disaccharide (Neu5Acalpha2-6GalNAcalpha1-O-Ser/Thr) cannot be further elongated and sialyl-Tn expression results therefore in a shortening of the O-glycan chains
  • Alternative names
    • CD175s antibody
    • Sialosyl Tn antibody
    • sTn antibody

Anti-Sialyl Tn antibody [STn 219] (FITC) images

  • Overlay histogram showing DU145 cells stained with ab76756 (red line). The cells were fixed with 80%methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76756, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed.

References for Anti-Sialyl Tn antibody [STn 219] (FITC) (ab76756)

ab76756 has not yet been referenced specifically in any publications.

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