Overview

  • Product nameAnti-SIDT1 antibody
    See all SIDT1 primary antibodies
  • Description
    Goat polyclonal to SIDT1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity

    Predicted to work with: Mouse, Rat, Horse, Cow, Human, Chimpanzee
  • Immunogen

    Synthetic peptide:

    C-QEKENQTWNLQRKKN

    , corresponding to amino acids 278-292 of Human SIDT1 (NP_060169.2).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/32,000.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 94 kDa.
    Preliminary experiments gave an approx 50kDa band in Human Brain (Cerebellum) and Human Breast lysates after 0.5µg/ml antibody staining. The 50kDa band was successfully blocked by incubation with the immunizing peptide.
    Please note that currently we cannot find an explanation in the literature for the band we observe.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    References for Anti-SIDT1 antibody (ab77429)

    ab77429 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab77429.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"