The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/32,000.
WB: Use at an assay dependent dilution. Predicted molecular weight: 94 kDa.
Preliminary experiments gave an approx 50kDa band in Human Brain (Cerebellum) and Human Breast lysates after 0.5µg/ml antibody staining. The 50kDa band was successfully blocked by incubation with the immunizing peptide.
Please note that currently we cannot find an explanation in the literature for the band we observe.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.