Overview

  • Product nameAnti-SIGLEC H antibody [440c]
  • Description
    Rat monoclonal [440c] to SIGLEC H
  • Tested applicationsSuitable for: IHC-Fr, Flow Cyt, Inhibition Assaymore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Purified bone-marrow derived mouse IPC

  • General notes


    Incubation of interferon producing cells with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing interferon producing cells depletion.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab106174 was 0.2 µm filtered prior to formulation.
  • Primary antibody notesIncubation of interferon producing cells with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing interferon producing cells depletion.
  • ClonalityMonoclonal
  • Clone number440c
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
Flow Cyt 1/50. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Inhibition Assay Use at an assay dependent concentration. (For inhibition of IFN-alpha secretion dilutions have to be made according to the amounts of SIGLEC H to be inactivated).

Target

  • RelevanceSIGLEC H a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo. The monoclonal antibody 440c exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • sialic acid binding Ig like lectin H antibody
    • SIGLEC like protein antibody
    • Siglech antibody

References for Anti-SIGLEC H antibody [440c] (ab106174)

This product has been referenced in:
  • Blasius AL  et al. Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12. Blood 107:2474-6 (2006). Read more (PubMed: 16293595) »
  • Blasius A  et al. A cell-surface molecule selectively expressed on murine natural interferon-producing cells that blocks secretion of interferon-alpha. Blood 103:4201-6 (2004). Read more (PubMed: 14695235) »

See all 2 Publications for this product

Product Wall

Merci de votre intérêt pour ab106174. Comme discuté au téléphone, cet anticorps n’a pas encore été testé IHC en coupes paraffine (IHC-P) à notre conaissance. Cependant, si vous souhaitez tester ab106174 IHC-P, il me sera possible de vous offrir une rem...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"