Anti-SIGLEC11 antibody (ab106390)
Key features and details
- Rabbit polyclonal to SIGLEC11
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SIGLEC11 antibody
See all SIGLEC11 primary antibodies -
Description
Rabbit polyclonal to SIGLEC11 -
Host species
Rabbit -
Specificity
Specific to Human SIGLEC11, does not cross react with SIGLEC10. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
18 amino acid peptide from near the carboxy terminus of Human SIGLEC11 (NP_443116).
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Positive control
- HepG2 cell lysate Human liver tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 76 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 76 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,8-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. -
Tissue specificity
Expressed by macrophages in various tissues including Kupffer cells. Also found in brain microglia. -
Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Post-translational
modificationsPhosphorylated on tyrosine residues. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 114132 Human
- Omim: 607157 Human
- SwissProt: Q96RL6 Human
- Unigene: 661852 Human
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Alternative names
- sialic acid binding Ig like lectin 11 antibody
- sialic acid binding lectin 11 antibody
- Sialic acid-binding Ig-like lectin 11 antibody
see all
Images
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ab106390 at 5µg/ml, staining SIGLEC11 in Human liver, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
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Immunofluorescence of SIGLEC11 in Human liver tissue with ab106390 at 20 ug/mL.
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Anti-SIGLEC11 antibody (ab106390) at 1 µg/ml + HepG2 cell lysate at 15 µg
Predicted band size: 76 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab106390 has not yet been referenced specifically in any publications.