Overview

  • Product nameSIGLEC5 Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 8 pg/ml
  • Range
    6.86 pg/ml - 5000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101.3 91% - 108%
    Serum 106.3 93% - 114%
    Plasma 102.4 91% - 111%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s SIGLEC5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SIGLEC5 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human SIGLEC5 coated on a 96-well plate. Standards and samples are pipetted into the wells and SIGLEC5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SIGLEC5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • RelevancePutative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differenciation.
  • Alternative names
    • CD170
    • CD170 antigen
    • CD33 antigen like 2
    • CD33L2
    • mSiglec F
    • OB binding protein 2
    • OB BP2
    • OBBP2
    • Obesity binding protein 2
    • OTTMUSP00000041735
    • OTTMUSP00000041736
    • Sialic acid binding Ig like lectin 5
    • Sialic acid binding immunoglobulin like lectin 5
    • SIGLEC 5
    • Siglec F
    • Siglec F variant
    • Siglecf
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

SIGLEC5 Human ELISA Kit images

  • Representative standard curve using ab100641

  • Representative standard curve using ab100641

Protocols

References for SIGLEC5 Human ELISA Kit (ab100641)

ab100641 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"