Overview

  • Product nameAnti-SIGLEC8 antibody
    See all SIGLEC8 primary antibodies
  • Description
    Rabbit polyclonal to SIGLEC8
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (10-30 amino acids in length) conjugated to KLH, selected from within amino acids 413-431 of Human SIGLEC8.

  • Positive control
    • HL60 cell lysate, Mouse liver tissue lysate, Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab38578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionPutative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
  • Tissue specificityExpressed specifically on eosinophils.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • DomainContains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD329 antigen antibody
    • CDw329 antibody
    • SAF 2 antibody
    • SAF-2 antibody
    • SAF2 antibody
    • Sialic acid binding Ig like lectin 8 antibody
    • Sialic acid-binding Ig-like lectin 8 antibody
    • Sialoadhesin family member 2 antibody
    • SIGL8_HUMAN antibody
    • Siglec 8 antibody
    • Siglec-8 antibody
    • SIGLEC8 antibody
    • SIGLEC8L antibody
    see all

Anti-SIGLEC8 antibody images

  • All lanes : Anti-SIGLEC8 antibody (ab38578) at 1/100 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : HL60 cell lysate


    Predicted band size : 54 kDa
    Observed band size : 54 kDa
    Additional bands at : 14 kDa,37 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • ab38578 staining SIGLEC8 in Human breast carcinoma by Immunohistochemistry, Formalin fixed, Paraffin embedded tissue. ab38578 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References for Anti-SIGLEC8 antibody (ab38578)

ab38578 has not yet been referenced specifically in any publications.

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In der Tat haben wir den Anti-SIGLEC8-Antikörper ab38578 in der IHC an Paraffin-eingebetteten Schnitten (Mammakarzinom, Hepatokarzinom) getestet und können garantieren, dass er in dieser Anwendung funktioniert, ...

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