Overview

  • Product name
    Anti-SIGLEC9 antibody [MM0552-6K12]
    See all SIGLEC9 primary antibodies
  • Description
    Mouse monoclonal [MM0552-6K12] to SIGLEC9
  • Tested applications
    Suitable for: ICC/IF, WB, Neutralisingmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment of Human SIGLEC9 extracellular domain.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and 0.2 µm filtered.
  • Clonality
    Monoclonal
  • Clone number
    MM0552-6K12
  • Isotype
    IgG2
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 25673840
WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.
Neutralising Use at an assay dependent concentration.

Target

  • Function
    Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
  • Tissue specificity
    Expressed by peripheral blood leukocytes (neutrophils and monocytes but not eosinophils). Found in liver, fetal liver, bone marrow, placenta, spleen and in lower levels in skeletal muscle, fetal brain, stomach, lung, thymus, prostate, brain, mammary, adrenal gland, colon, trachea, cerebellum, testis, small intestine and spinal cordon.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Domain
    Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD329 antibody
    • CDw329 antibody
    • FOAP 9 antibody
    • OB binding protein like antibody
    • OBBP LIKE antibody
    • Protein FOAP 9 antibody
    • Protein FOAP-9 antibody
    • sialic acid binding Ig like lectin 9 antibody
    • Sialic acid-binding Ig-like lectin 9 antibody
    • Sialic acid-binding immunoglobulin like lectin 9 antibody
    • SIGL9_HUMAN antibody
    • Siglec 9 antibody
    • Siglec-9 antibody
    • SIGLEC9 antibody
    see all

References for Anti-SIGLEC9 antibody [MM0552-6K12] (ab89484)

This product has been referenced in:
  • Matsubara K  et al. Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity. J Neurosci 35:2452-64 (2015). ICC/IF ; Rat . Read more (PubMed: 25673840) »
  • Brazil JC  et al. a3/4 Fucosyltransferase 3-dependent synthesis of Sialyl Lewis A on CD44 variant containing exon 6 mediates polymorphonuclear leukocyte detachment from intestinal epithelium during transepithelial migration. J Immunol 191:4804-17 (2013). Read more (PubMed: 24068663) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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