Overview

  • Product nameAnti-Sigma 32 antibody
  • Description
    Rabbit polyclonal to Sigma 32
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with Serratia marcescens. Not yet tested in other species.
  • Immunogen

    Full length Sigma 32 protein (Serratia marcescens)

Properties

Applications

Our Abpromise guarantee covers the use of ab26890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).

Target

  • RelevanceWhen cells are exposed to stresses such as heat or ethanol, a set of well-conserved heat shock proteins (HSPs) are rapidly induced. Induction of HSPs is controlled primarily at the level of transcription. In Serratia marcescens, expression of HSPs is positively regulated by a heat shock transcription factor-sigma 32 encoded by the rpoH gene. The rPOH protein plays a major role in regulation of the heat shock response by enhancing transcription of the heat shock genes.
  • Alternative names
    • RNA polymerase sigma 32 factor antibody
    • rpo H antibody
    • rpoH antibody

Anti-Sigma 32 antibody images

  • Lane 1 : Pre-bleed at 1/2000 dilution
    Lane 2 : Anti-serum at 1/2000 dilution

    Lane 1 : Antigen
    Lane 2 : Antigen

    Lysates/proteins at 4 µg per lane.

    Secondary
    anti-rabbit IgG-HRP
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-Sigma 32 antibody (ab26890)

ab26890 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10.9912 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Escherichia coli Cell lysate - whole cell (no specification)
Specification no specification
Treatment 100 ´M Nalidixic Acid for 18 hours
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Feb 18 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"