Anti-Sigma1-receptor antibody (ab223702)
Key features and details
- Rabbit polyclonal to Sigma1-receptor
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Sigma1-receptor antibody
See all Sigma1-receptor primary antibodies -
Description
Rabbit polyclonal to Sigma1-receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Guinea pig, Cow -
Immunogen
Recombinant fragment corresponding to Human Sigma1-receptor aa 1-100.
Database link: Q99720 -
Positive control
- WB: HEK-293T, Caco-2 and HeLa cell lysate. IHC-P: Human liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 25 kDa.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 25 kDa. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Functions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration. -
Tissue specificity
Widely expressed with higher expression in liver, colon, prostate, placenta, small intestine, heart and pancreas. Expressed in the retina by retinal pigment epithelial cells. -
Involvement in disease
Amyotrophic lateral sclerosis 16, juvenile -
Sequence similarities
Belongs to the ERG2 family. -
Cellular localization
Nucleus inner membrane. Nucleus outer membrane. Endoplasmic reticulum membrane. Lipid droplet. Cell junction. Cell membrane. Cell projection > growth cone. Targeted to lipid droplets, cholesterol and galactosylceramide-enriched domains of the endoplasmic reticulum. Enriched at cell-cell communication regions, growth cone and postsynaptic structures. Localization is modulated by ligand-binding. - Information by UniProt
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Database links
- Entrez Gene: 538903 Cow
- Entrez Gene: 100135589 Guinea pig
- Entrez Gene: 10280 Human
- Entrez Gene: 18391 Mouse
- Entrez Gene: 29336 Rat
- Omim: 601978 Human
- SwissProt: Q58DH7 Cow
- SwissProt: Q60492 Guinea pig
see all -
Alternative names
- Aging associated gene 8 protein antibody
- Aging-associated gene 8 protein antibody
- AL024364 antibody
see all
Images
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All lanes : Anti-Sigma1-receptor antibody (ab223702) at 0.04 µg/ml
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : SIGMAR1 knockout HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab223702 observed at 24 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab223702 was shown to react with Sigma1-receptor in wild-type HEK-293T cells in Western blot with loss of signal observed in SIGMAR1 knockout cell line ab266619 (SIGMAR1 knockout cell lysate ab258666). Wild-type HEK-293T and SIGMAR1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab223702 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at 0.04 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Immunohistochemical analysis of Formalin fixed, Paraffin-embedded human liver tissue labeling Sigma 1-receptor with ab223702 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of Formalin fixed, Paraffin-embedded human cerebellum tissue labeling Sigma 1-receptor with ab223702 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of Formalin fixed, Paraffin-embedded human Fallopian tube tissue labeling Sigma 1-receptor with ab223702 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of Formalin fixed, Paraffin-embedded human gastrointestinal tissue labeling Sigma 1-receptor with ab223702 at 1/20 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-Sigma1-receptor antibody (ab223702) at 1/100 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 25 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223702 has not yet been referenced specifically in any publications.