Anti-Sigma1-receptor antibody - C-terminal (ab53852)

Overview

  • Product name
    Anti-Sigma1-receptor antibody - C-terminal
    See all Sigma1-receptor primary antibodies
  • Description
    Rabbit polyclonal to Sigma1-receptor - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-FrFl, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Dog, Human, Mink
  • Immunogen

    Synthetic peptide corresponding to Rat Sigma1-receptor (C terminal).
    Database link: Q99720

  • Positive control
    • WB: Mouse brain, rat liver and rat brain homogenates. IHC-Free Floating: Mouse lumbar spinal cord tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab53852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-Fr 1/200.
IHC-FrFl 1/500.
ICC/IF Use at an assay dependent concentration. PubMed: 21573736

Target

  • Function
    Functions in lipid transport from the endoplasmic reticulum and is involved in a wide array of cellular functions probably through regulation of the biogenesis of lipid microdomains at the plasma membrane. Involved in the regulation of different receptors it plays a role in BDNF signaling and EGF signaling. Also regulates ion channels like the potassium channel and could modulate neurotransmitter release. Plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. Plays a role in several other cell functions including proliferation, survival and death. Originally identified for its ability to bind various psychoactive drugs it is involved in learning processes, memory and mood alteration.
  • Tissue specificity
    Widely expressed with higher expression in liver, colon, prostate, placenta, small intestine, heart and pancreas. Expressed in the retina by retinal pigment epithelial cells.
  • Involvement in disease
    Amyotrophic lateral sclerosis 16, juvenile
  • Sequence similarities
    Belongs to the ERG2 family.
  • Cellular localization
    Nucleus inner membrane. Nucleus outer membrane. Endoplasmic reticulum membrane. Lipid droplet. Cell junction. Cell membrane. Cell projection > growth cone. Targeted to lipid droplets, cholesterol and galactosylceramide-enriched domains of the endoplasmic reticulum. Enriched at cell-cell communication regions, growth cone and postsynaptic structures. Localization is modulated by ligand-binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aging associated gene 8 protein antibody
    • Aging-associated gene 8 protein antibody
    • AL024364 antibody
    • hSigmaR1 antibody
    • mSigmaR1 antibody
    • opioid receptor, sigma 1 antibody
    • opioid receptor, sigma 1 isoform 1 antibody
    • RP23 167I12.6 antibody
    • SGMR1_HUMAN antibody
    • SIG 1R antibody
    • SIG-1R antibody
    • Sigma 1-type opioid receptor antibody
    • Sigma non-opioid intracellular receptor 1 antibody
    • sigma1 receptor antibody
    • Sigma1-receptor antibody
    • Sigma1R antibody
    • Sigmar1 antibody
    • SR BP antibody
    • SR-BP antibody
    • SR31747 binding protein antibody
    • SR31747-binding protein antibody
    • SRBP antibody
    see all

Images

  • All lanes : Anti-Sigma1-receptor antibody - C-terminal (ab53852) at 2 µg/ml

    Lane 1 : Mouse brain homogenate
    Lane 2 : Rat liver homogenate
    Lane 3 : Rat brain homogenate

    Predicted band size: 25 kDa
    Observed band size: 25 kDa
    Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.

  • ab53852 staining Sigma1-receptor in mouse lumbar spinal cord tissue (30 micron sections) by Immunohistochemistry (Free-floating sections). Blocking was done by incubating the sections for 1 hour at room temperature in NGS 10% + Triton 0.1% in PBS. The primary antibody was diluted 1/250 in 3% goat serum in PBS and incubated with the sample for 16 hours at 4°C. An Alexa fluor® 647-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/500.
    Sections were also immunostained with Hoechst dye (blue) to highlight nuclei. The green signal is GFP expressed in motor neuronal cells.

    See Abreview

References

This product has been referenced in:
  • Feltmann K  et al. Effects of Long-Term Alcohol Drinking on the Dopamine D2 Receptor: Gene Expression and Heteroreceptor Complexes in the Striatum in Rats. Alcohol Clin Exp Res 42:338-351 (2018). Read more (PubMed: 29205397) »
  • Lencesova L  et al. Disruption of dopamine D1/D2 receptor complex is involved in the function of haloperidol in cardiac H9c2 cells. Life Sci 191:186-194 (2017). ICC/IF . Read more (PubMed: 29054453) »

See all 14 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (SH-SY5Y cells)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
60 µg
Specification
SH-SY5Y cells
Blocking step
BlockingOne as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 04 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (hypothalamus PVN sections)
Specification
hypothalamus PVN sections
Fixative
70% ethanol
Permeabilization
Yes - triton-x
Blocking step
2% BSA 2% NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Lumbar Spinal Cord section (30 micron thickness))
Specification
Lumbar Spinal Cord section (30 micron thickness)
Username

Dr. Marco Peviani

Verified customer

Submitted Feb 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Cerebral)
Loading amount
50 µg
Specification
Cerebral
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 31 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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