Overview

  • Product name
    Anti-SIGN Related 1 antibody [ER-TR9] (Biotin)
    See all SIGN Related 1 primary antibodies
  • Description
    Rat monoclonal [ER-TR9] to SIGN Related 1 (Biotin)
  • Conjugation
    Biotin
  • Specificity
    Reacts with subpopulation of mature tissue macrophages present in the splenic marginal zone, lymph node medullary and trabecular sinuses.
  • Tested applications
    Suitable for: IHC-Frmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse thymus cells

  • Epitope
    The antigen is a glutaraldehyde (0.05%) resistant protein expressed in the cytoplasm and on the cell surface.
  • Positive control
    • Mouse spleen.
  • General notes
    The antigen recognized by ER-TR9 has recently been shown to be the murine analogue of the human DC SIGN (Dendritic Cell - Specific ICAM3 Grabbing Non Integrin), named SIGN R1. Antigen Distribution: Isolated Cells: The antigen is found on a subpopulation of phagocytic macrophages isolated from the spleen and showing acid phosphatase and moderate non-specific esterase activity. These phagocytes selectively ingest neutral polysaccharides such as Ficoll. Tissue Sections: Subpopulation of resident macrophages in the splenic marginal zone which are in the proximity of a certain B cell subpopulation (µ+, d-). It is also found on a subpopulation of macrophages localized in the medullary sinuses and trabecular sinuses of lymph nodes. Furthermore, macrophage subpopulations in other organs, such as some connective tissue macrophages in the dermis, may also show ER-TR9 antigen expression.


    Monoclonal antibody ER-TR9 is a very useful marker for the identification of macrophage subpopulations present in the marginal zone of spleen and lymph node medulla. ER-TR9 is also useful when studying phagocytosis of polysaccharides since the antibody selectively inhibits uptake of these glycans by macrophages.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, 5mg/ml BSA, pH 7.2
  • Concentration information loading...
  • Purity
    Purified IgM
  • Primary antibody notes
    Monoclonal antibody ER-TR9 is a very useful marker for the identification of macrophage subpopulations present in the marginal zone of spleen and lymph node medulla. ER-TR9 is also useful when studying phagocytosis of polysaccharides since the antibody selectively inhibits uptake of these glycans by macrophages.
  • Clonality
    Monoclonal
  • Clone number
    ER-TR9
  • Isotype
    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 1.5 µg/ml.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Relevance
      SIGN R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in some strains, in the peritoneal cavity. MZM of the spleen are involved in the clearance of polysaccharides. Mouse SIGN R1 is a C type lectin, like DC SIGN which is expressed on Human dendritic cells (DCs). However, Mmouse SIGN R1 itself is not expressed on DCs. SIGN R1 exists in an aggregated form, resistant to dissociation into monomers upon boiling in SDS under reducing conditions. SIGN R1 mediates the uptake of encapsulated organisms and may be an important mediator for the uptake of microbes in both spleen and lymph node, particularly through the recognition of microbial polysaccharides.
    • Cellular localization
      Single-pass type II membrane protein
    • Database links
    • Alternative names
      • CD209 antigen like protein B antibody
      • CD209b antigen antibody
      • DC SIGN related protein 1 antibody
      • DC SIGNR1 antibody
      • OtB7 antibody
      see all

    References

    This product has been referenced in:
    • Kang YS  et al. SIGN-R1, a novel C-type lectin expressed by marginal zone macrophages in spleen, mediates uptake of the polysaccharide dextran. Int Immunol 15:177-86 (2003). Read more (PubMed: 12578847) »
    • Kraal G  et al. Marginal zone macrophages and their role in the immune response against T-independent type 2 antigens: modulation of the cells with specific antibody. Eur J Immunol 19:675-80 (1989). Read more (PubMed: 2471648) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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