EpitopeThe antigen is a glutaraldehyde (0.05%) resistant protein expressed in the cytoplasm and on the cell surface.
General notesThe antigen recognized by ER-TR9 has recently been shown to be the murine analogue of the human DC SIGN (Dendritic Cell - Specific ICAM3 Grabbing Non Integrin), named SIGN R1.
Isolated Cells: The antigen is found on a subpopulation of phagocytic macrophages isolated from the spleen and showing acid phosphatase and moderate non-specific esterase activity. These phagocytes selectively ingest neutral polysaccharides such as Ficoll.
Tissue Sections: Subpopulation of resident macrophages in the splenic marginal zone which are in the proximity of a certain B cell subpopulation (µ+, d-). It is also found on a subpopulation of macrophages localized in the medullary sinuses and trabecular sinuses of lymph nodes. Furthermore, macrophage subpopulations in other organs, such as some connective tissue macrophages in the dermis, may also show ER-TR9 antigen expression.
Monoclonal antibody ER-TR9 is a very useful marker for the identification of macrophage subpopulations present in the marginal zone of spleen and lymph node medulla. ER-TR9 is also useful when studying phagocytosis of polysaccharides since the antibody selectively inhibits uptake of these glycans by macrophages.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Primary antibody notesMonoclonal antibody ER-TR9 is a very useful marker for the identification of macrophage subpopulations present in the marginal zone of spleen and lymph node medulla. ER-TR9 is also useful when studying phagocytosis of polysaccharides since the antibody selectively inhibits uptake of these glycans by macrophages.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.5 µg/ml.
Application notesIs unsuitable for IHC-P.
RelevanceSIGN R1 is a specific marker for the identification of macrophage subpopulations present in the marginal zone of spleen (the so-called marginal zone macrophages (MZM)), in the lymph node medulla, and in some strains, in the peritoneal cavity. MZM of the spleen are involved in the clearance of polysaccharides.
Mouse SIGN R1 is a C type lectin, like DC SIGN which is expressed on Human dendritic cells (DCs). However, Mmouse SIGN R1 itself is not expressed on DCs. SIGN R1 exists in an aggregated form, resistant to dissociation into monomers upon boiling in SDS under reducing conditions.
SIGN R1 mediates the uptake of encapsulated organisms and may be an important mediator for the uptake of microbes in both spleen and lymph node, particularly through the recognition of microbial polysaccharides.
Cellular localizationSingle-pass type II membrane protein
References for Anti-SIGN Related 1 antibody [ER-TR9] (Biotin) (ab51819)
This product has been referenced in:
Kang YS et al. SIGN-R1, a novel C-type lectin expressed by marginal zone macrophages in spleen, mediates uptake of the polysaccharide dextran. Int Immunol15:177-86 (2003).
Read more (PubMed: 12578847) »
Kraal G et al. Marginal zone macrophages and their role in the immune response against T-independent type 2 antigens: modulation of the cells with specific antibody. Eur J Immunol19:675-80 (1989).
Read more (PubMed: 2471648) »