Anti-Signal peptide peptidase-like 2B antibody (ab43856)

Overview

  • Product nameAnti-Signal peptide peptidase-like 2B antibody
    See all Signal peptide peptidase-like 2B primary antibodies
  • Description
    Rabbit polyclonal to Signal peptide peptidase-like 2B
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Signal peptide peptidase-like 2B.

    (Peptide available as ab43855.)

  • Positive control
    • This antibody gave a positive signal in WI38 Whole Cell Lysate, Human Thymus Tissue Lysate and Human Brain Tissue Lysate (data not shown).

Properties

Applications

Our Abpromise guarantee covers the use of ab43856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 65 kDa).

Target

  • RelevanceThe SPPLs are a group of aspartic proteases that are responsible for proteolysis of type II membrane-anchored signalling factors. SPPL2b, unlike other SPPL family members is found in endosomes/lysosomes, and not the endoplasmic reticulum, and has a role in catalysing intramembrane cleavage of tumour necrosis factor alpha (TNFalpha).
  • Cellular localizationMembrane; multi-pass membrane protein of endosomes/lysosomes.
  • Database links
  • Alternative names
    • IMP4 antibody
    • Intramembrane protease 4 antibody
    • Protein SPP-like 2B antibody
    • SPPL2B antibody

Anti-Signal peptide peptidase-like 2B antibody images

  • All lanes : Anti-Signal peptide peptidase-like 2B antibody (ab43856) at 1 µg/ml

    Lane 1 : WI-38 whole cell lysate (ab3960)
    Lane 2 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-Signal peptide peptidase-like 2B antibody (ab43856)

ab43856 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab43856.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"