The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 65 kDa).
The SPPLs are a group of aspartic proteases that are responsible for proteolysis of type II membrane-anchored signalling factors. SPPL2b, unlike other SPPL family members is found in endosomes/lysosomes, and not the endoplasmic reticulum, and has a role in catalysing intramembrane cleavage of tumour necrosis factor alpha (TNFalpha).
Membrane; multi-pass membrane protein of endosomes/lysosomes.
This antibody was raised against an immunogen that is predicted to recognize isoforms (1,2,3,4) of Signal peptide peptidase-like 2B. The predicted molecular weights of isoforms (1,2,3,4) are 65 kDa, 35 kDa, 34 kDa and 56 kDa respectively. The band observed at 37 kDa may represent isoform 2 of Signal peptide peptidase-like 2B.