Anti-SIK3 antibody (ab88495)

Overview

  • Product nameAnti-SIK3 antibody
    See all SIK3 primary antibodies
  • Description
    Rabbit polyclonal to SIK3
  • Tested applicationsSuitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide mapping to a region between residue 900 and 950 of human SIK3 using the numbering given in entry NP_079440.2

  • Positive control
    • Whole cell lysate from HeLa, 293T or NIH3T3 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 140 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceActivated by phosphorylation on Thr 163 by STK11 in complex with STE20 related adapter alpha (STRAD alpha) pseudo kinase and CAB39. Binds to and is activated by YWHAZ when phosphorylated on Thr 163.
  • Cellular localizationCytoplasm
  • Database links
  • Alternative names
    • FLJ12240 antibody
    • KIAA0999 antibody
    • L19 antibody
    • QSK antibody
    • Salt inducible kinase 3 antibody
    • serine/threonine-protein kinase QSK antibody
    • Serine/threonine-protein kinase SIK3 antibody
    • SIK 3 antibody
    • SIK family kinase 3 antibody
    • SIK3_HUMAN antibody
    see all

Anti-SIK3 antibody images

  • All lanes : Anti-SIK3 antibody (ab88495) at 0.4 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : NIH3T3 cell lysate at 50 µg


    Predicted band size : 140 kDa


    Exposure time : 30 seconds
  • 3ug ab88495 were used to immunoprecipitate 1mg HeLa whole cell lysate. 20% of immunoprecipitate was loaded on gel and probed with ab88495 at 1ug/ml.
    Detection: chemoluminescence with exposure time of 3 minutes.
  • ICC/IF image of ab88495 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88495, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-SIK3 antibody (ab88495)

ab88495 has not yet been referenced specifically in any publications.

Product Wall

Unfortunately, we do not have the corresponding blocking peptide available to buy in the catalogue.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 50 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jul 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"