Anti-SIK3 antibody (ab88495)
Key features and details
- Rabbit polyclonal to SIK3
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SIK3 antibody
See all SIK3 primary antibodies -
Description
Rabbit polyclonal to SIK3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human SIK3.
Database link: Q9Y2K2 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 140 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 140 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
Post-translational
modificationsPhosphorylated at Thr-163 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. -
Cellular localization
Cytoplasm. Locates to punctate structures within the cytoplasm on binding to YWHAZ. - Information by UniProt
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Database links
- Entrez Gene: 23387 Human
- Entrez Gene: 70661 Mouse
- Omim: 614776 Human
- SwissProt: Q9Y2K2 Human
- SwissProt: Q6P4S6 Mouse
- Unigene: 167451 Human
- Unigene: 219459 Mouse
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Alternative names
- FLJ12240 antibody
- KIAA0999 antibody
- L19 antibody
see all
Images
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All lanes : Anti-SIK3 antibody (ab88495) at 0.4 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : NIH3T3 cell lysate at 50 µg
Predicted band size: 140 kDa
Exposure time: 30 seconds -
3ug ab88495 were used to immunoprecipitate 1mg HeLa whole cell lysate. 20% of immunoprecipitate was loaded on gel and probed with ab88495 at 1ug/ml.
Detection: chemoluminescence with exposure time of 3 minutes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab88495 has been referenced in 2 publications.
- Tang CC et al. Dual targeting of salt inducible kinases and CSF1R uncouples bone formation and bone resorption. Elife 10:N/A (2021). PubMed: 34160349
- Sahin HB et al. Salt inducible kinases as novel Notch interactors in the developing Drosophila retina. PLoS One 15:e0234744 (2020). PubMed: 32542037