Overview

  • Product name
  • Description
    Rabbit polyclonal to SIKE1
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence: AEPVLKAHQS HSAEIESQID RICEMGEVMR KAVQVDDDQF CKIQEKLAQL ELENKELREL LSISSESLQA RKENSMD, corresponding to C terminal amino acids 124-200 of Human SIKE1.

  • Positive control
    • Human rectum tissue; Human liver and tonsil lysates; RT 4 and U 251 MG cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways.
  • Tissue specificity
    Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and leukocytes. Present in all cell lines tested (at protein level).
  • Sequence similarities
    Belongs to the SIKE family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ21168 antibody
    • RGD1311316 antibody
    • RP5-1000E10.4 antibody
    • SIKE antibody
    • sike1 antibody
    • SIKE1_HUMAN antibody
    • Suppressor of IKBKE 1 antibody
    • Suppressor of IKK-epsilon antibody
    see all

Anti-SIKE1 antibody images

  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • ab121860, at 1/150 dilution, staining SIKE1 in glandular cells of paraffin-embedded Human rectum tissue by Immunohistochemistry.
  • All lanes : Anti-SIKE1 antibody (ab121860) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References for Anti-SIKE1 antibody (ab121860)

ab121860 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab121860.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up