Overview

  • Product nameAnti-SIL1 antibody [1C4]
    See all SIL1 primary antibodies
  • Description
    Mouse monoclonal [1C4] to SIL1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein of Human SIL1 produced in HEK293T cells (NP_071909).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY SIL1 cDNA, SIL1 transiently transfected COS7 cells, Human kidney, liver, carcinoma of liver, Adenocarcinoma of ovary, pancreas, and Carcinoma of prostate tissues
  • General notesDilute in PBS (pH7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab118155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 52 kDa.
IHC-P 1/50.
ICC/IF 1/100.

Target

  • FunctionRequired for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.
  • Tissue specificityHighly expressed in tissues which produce large amounts of secreted proteins such as kidney, liver and placenta. Also expressed in colon, heart, lung, ovary, pancreas, peripheral leukocyte, prostate, spleen and thymus. Expressed at low levels throughout the brain.
  • Involvement in diseaseDefects in SIL1 are a cause of Marinesco-Sjoegren syndrome (MSS) [MIM:248800]. MSS is an autosomal recessive multisystem disorder which is characterized by cerebellar ataxia due to cerebellar atrophy, with Purkinje and granule cell loss and myopathy featuring marked muscle replacement with fat and connective tissue. Other cardinal features include bilateral cataracts, hypergonadotrophic hypogonadism and mild to severe mental retardation. Skeletal abnormalities, short stature, dysarthria, strabismus and nystagmus are also frequent findings. Mutational inactivation of this protein may result in ER stress-induced cell death signaling or malfunctioning chaperone machineries that mishandle client proteins which are critical for the organs targeted in MSS.
  • Sequence similaritiesBelongs to the SIL1 family.
  • Developmental stageExpressed in fetal kidney, fetal lung, fetal liver and at low levels in fetal brain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationEndoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAP antibody
    • BiP associated protein antibody
    • BiP-associated protein antibody
    • Endoplasmic reticulum chaperone SIL 1 antibody
    • Endoplasmic reticulum chaperone SIL1 antibody
    • MSS antibody
    • Nucleotide exchange factor SIL 1 antibody
    • Nucleotide exchange factor SIL1 antibody
    • SIL 1 antibody
    • sil1 antibody
    • SIL1 homolog antibody
    • SIL1 homolog endoplasmic reticulum chaperone (S. cerevisiae) antibody
    • SIL1 homolog endoplasmic reticulum chaperone antibody
    • SIL1_HUMAN antibody
    • UGL 5 antibody
    • ULG5 antibody
    see all

Anti-SIL1 antibody [1C4] images

  • All lanes : Anti-SIL1 antibody [1C4] (ab118155) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SIL1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 52 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211850)
  • Immunohistochemical staining of paraffin-embedded Human Kidney tissue using ab118155 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin-embedded Human liver tissue using ab118155 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin-embedded Human carcinoma of liver tissue using ab118155 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin-embedded Human Adenocarcinoma of ovary tissue using ab118155 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin-embedded Human pancreas tissue using ab118155 at a dilution of 1/50.
  • Immunohistochemical staining of paraffin-embedded Human carcinoma of prostate tissue using ab118155 at a dilution of 1/50.
  • Immunofluorescent staining of COS7 cells transiently transfected with SIL1 using ab118155 at a dilution of 1/100.

References for Anti-SIL1 antibody [1C4] (ab118155)

ab118155 has not yet been referenced specifically in any publications.

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