Overview

  • Product nameAnti-SIL1 antibody
    See all SIL1 primary antibodies
  • Description
    Mouse polyclonal to SIL1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human SIL1 protein (NP_071909.1).

  • Positive control
    • HeLa cell lysate, LNCaP cell lysate and SIL1 transfected 293T cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab89678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa.

Target

  • FunctionRequired for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.
  • Tissue specificityHighly expressed in tissues which produce large amounts of secreted proteins such as kidney, liver and placenta. Also expressed in colon, heart, lung, ovary, pancreas, peripheral leukocyte, prostate, spleen and thymus. Expressed at low levels throughout the brain.
  • Involvement in diseaseDefects in SIL1 are a cause of Marinesco-Sjoegren syndrome (MSS) [MIM:248800]. MSS is an autosomal recessive multisystem disorder which is characterized by cerebellar ataxia due to cerebellar atrophy, with Purkinje and granule cell loss and myopathy featuring marked muscle replacement with fat and connective tissue. Other cardinal features include bilateral cataracts, hypergonadotrophic hypogonadism and mild to severe mental retardation. Skeletal abnormalities, short stature, dysarthria, strabismus and nystagmus are also frequent findings. Mutational inactivation of this protein may result in ER stress-induced cell death signaling or malfunctioning chaperone machineries that mishandle client proteins which are critical for the organs targeted in MSS.
  • Sequence similaritiesBelongs to the SIL1 family.
  • Developmental stageExpressed in fetal kidney, fetal lung, fetal liver and at low levels in fetal brain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationEndoplasmic reticulum lumen.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAP antibody
    • BiP associated protein antibody
    • BiP-associated protein antibody
    • Endoplasmic reticulum chaperone SIL 1 antibody
    • Endoplasmic reticulum chaperone SIL1 antibody
    • MSS antibody
    • Nucleotide exchange factor SIL 1 antibody
    • Nucleotide exchange factor SIL1 antibody
    • SIL 1 antibody
    • sil1 antibody
    • SIL1 homolog antibody
    • SIL1 homolog endoplasmic reticulum chaperone (S. cerevisiae) antibody
    • SIL1 homolog endoplasmic reticulum chaperone antibody
    • SIL1_HUMAN antibody
    • UGL 5 antibody
    • ULG5 antibody
    see all

Anti-SIL1 antibody images

  • Anti-SIL1 antibody (ab89678) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Anti-SIL1 antibody (ab89678) at 1 µg/ml + LNCaP cell lysate at 50 µg

    Predicted band size : 52 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-SIL1 antibody (ab89678) at 1 µg/ml

    Lane 1 : SIL1 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa

References for Anti-SIL1 antibody (ab89678)

ab89678 has not yet been referenced specifically in any publications.

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