Overview

  • Product nameAnti-Silencer of Death Domain antibody
    See all Silencer of Death Domain primary antibodies
  • Description
    Rabbit polyclonal to Silencer of Death Domain
  • SpecificityHuman, Mouse, Rat.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    SALRRSGYGPSDGPSC

    , corresponding to amino acids 2-16 of Human SODD.

  • Positive control
    • HeLa whole cell lysate or THP-1 whole cell lysate.
  • General notesSilencer of Death Domains.


    Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identifiedand designated SODD for silencer of death domains. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPBS with 0.02% sodium azide
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesSODD Antibody is Antibody is DEAE purified.
  • Primary antibody notesApoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identifiedand designated SODD for silencer of death domains. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab2048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/2000. Detects a band of approximately 60 kDa.Can be blocked with Silencer of Death Domain peptide (ab8382).

Target

Anti-Silencer of Death Domain antibody images

  • All lanes : Anti-Silencer of Death Domain antibody (ab2048) at 1/500 dilution

    Lane 1 : HeLa whole cell lysate with absence of blocking peptide
    Lane 2 : THP-1 whole cell lysate with absence of blocking peptide
    Lane 3 : HeLa whole cell lysate with Silencer of Death Domain peptide (ab8382)
    Lane 4 : THP-1 whole cell lysate with Silencer of Death Domain peptide (ab8382)


    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab2048 at 5µg/ml staining Silencer of Death Domain in Hela cells by ICC/IF 

  • Immunofluorescence of SODD in Hela cells using ab2048 at 20 ug/ml.

References for Anti-Silencer of Death Domain antibody (ab2048)

This product has been referenced in:
  • Boiani M  et al. The stress protein BAG3 stabilizes Mcl-1 protein and promotes survival of cancer cells and resistance to antagonist ABT-737. J Biol Chem 288:6980-90 (2013). WB ; Human . Read more (PubMed: 23341456) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"