Anti-Silencer of Death Domain antibody [EPR3596] (ab108988)

Overview

  • Product nameAnti-Silencer of Death Domain antibody [EPR3596]
    See all Silencer of Death Domain primary antibodies
  • Description
    Rabbit monoclonal [EPR3596] to Silencer of Death Domain
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Silencer of Death Domain (UniProt O95429).

  • Positive control
    • WB: HeLa, HepG2, and Molt-4 cell lysates ICC/IF: HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 50 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IHC-P or IP.
  • Target

    • FunctionInhibits the chaperone activity of HSP70/HSC70 by promoting substrate release (By similarity). Prevents constitutive TNFRSF1A signaling.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesContains 1 BAG domain.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • BAG 4 antibody
      • BAG family molecular chaperone regulator 4 antibody
      • BAG-4 antibody
      • BAG4 antibody
      • BAG4_HUMAN antibody
      • Bcl 2 associated athanogene 4 antibody
      • Bcl-2-associated athanogene 4 antibody
      • BCL2 associated athanogene 4 antibody
      • Silencer of death domains antibody
      • SODD antibody
      see all

    Anti-Silencer of Death Domain antibody [EPR3596] images

    • All lanes : Anti-Silencer of Death Domain antibody [EPR3596] (ab108988) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Molt-4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 50 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of Silencer of Death Domain in HeLa cells, using ab108988 at a 1/100 dilution.

    References for Anti-Silencer of Death Domain antibody [EPR3596] (ab108988)

    ab108988 has not yet been referenced specifically in any publications.

    Product Wall

    The Anti-Silencer of Death Domain antibody ab108988 is unlikely to react with the sequence you provided. The immunogen for the antibody is a 15 amino acid peptide and alignment with your sequence returns only 4 matching amino acids, PPQ-P.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"