Silver Enhancer Kit (Light Insensitive) (ab170733)

Overview

  • Product name
    Silver Enhancer Kit (Light Insensitive)
  • Sample type
    Tissue, Suspension cells
  • Product overview

    Abcam’s Silver Enhancer (Light Insensitive) Kit (ab170733) is a convenient, light-insensitive silver enhancement system for use with gold particle immunoreagents, which may be used for light microscopy or to visualize gold particles on gels or blots. Silver Enhancer (Light Insensitive) is nucleated quickly by gold nanoparticles, resulting in the precipitation of metallic silver and the formation of a dark brown to black signal. The system has markedly delayed self-nucleation resulting in high contrast and very low background and non-specific staining. ab170733 consists of 125 mL ready-to-use enhancer and 125 mL ready-to-use initiator; the reagent is formed by combining equal volumes of these solutions. The mixture is usable only for a defined time period, as given below, and should therefore be prepared immediately before use. For electron microscopy, we recommend ab170732

  • Tested applications
    Suitable for: WB, Electron Microscopy, IHC, ICCmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab170733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
Electron Microscopy Use at an assay dependent concentration.
IHC Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Protocols

References

ab170733 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Staining gold nanoparticles

Good Good 4/5 (Ease of Use)
Abreviews
We used the kit to visualize gold nanoparticles conjugated to chemotherapeutic drugs in the kidney. The enhancement works very well, however in order to visualize the particles at normal light microscope magnifications the development time was increased to 60 minutes. This produced little background staining and distinct positive staining. We counterstained the tissue with neutral red.
Username

Ms. Katie Belsham

Verified customer

Submitted Mar 31 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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