Overview

  • Product name
    Anti-SIM1 antibody - N-terminal
    See all SIM1 primary antibodies
  • Description
    Rabbit polyclonal to SIM1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SIM1 aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P81133

  • Positive control
    • ZR-75-1 cell line lysate; Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab171412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 86 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transcriptional factor that may have pleiotropic effects during embryogenesis and in the adult.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
    Contains 1 Single-minded C-terminal domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHe14 antibody
    • Class E basic helix-loop-helix protein 14 antibody
    • SIM1 antibody
    • SIM1_HUMAN antibody
    • Single minded homolog 1 antibody
    • Single-minded homolog 1 antibody
    see all

Images

  • Anti-SIM1 antibody - N-terminal (ab171412) at 1/100 dilution + ZR-75-1 cell line lysate at 35 µg

    Predicted band size: 86 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling SIM1 with ab171412 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab171412 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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