Overview

  • Product nameAnti-SIN1 antibody
    See all SIN1 primary antibodies
  • Description
    Rabbit polyclonal to SIN1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 480-510 of Human SIN1
    (NP_001006622.1, NP_077022.1, NP_001006620.1, NP_001006618.1, NP_001006621.1, NP_001006619.1).

  • Positive control
    • MCF7 cell line lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab116286 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 59 kDa.

Target

  • FunctionSubunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.
  • Tissue specificityUbiquitously expressed, with highest levels in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the SIN1 family.
  • Cellular localizationCell membrane. Cytoplasmic vesicle. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAPKAP 1 antibody
    • MAPKAP1 antibody
    • MEKK2 interacting protein 1 antibody
    • MGC2745 antibody
    • MIP 1 antibody
    • MIP1 antibody
    • Mitogen activated protein kinase associated protein 1 antibody
    • Mitogen-activated protein kinase 2-associated protein 1 antibody
    • mSIN1 antibody
    • OTTHUMP00000064207 antibody
    • Ras inhibitor MGC2745 antibody
    • SAPK interacting protein 1 antibody
    • SAPK-interacting protein 1 antibody
    • SIN 1 antibody
    • SIN1_HUMAN antibody
    • SIN1b antibody
    • SIN1g antibody
    • Stress activated map kinase interacting protein 1 antibody
    • Stress activated protein kinase interacting 1 antibody
    • Stress-activated map kinase-interacting protein 1 antibody
    • Target of rapamycin complex 2 subunit MAPKAP1 antibody
    • TORC2 subunit MAPKAP1 antibody
    see all

Anti-SIN1 antibody images

  • Anti-SIN1 antibody (ab116286) at 1/100 dilution + MCF7 cell line lysate at 35 µg

    Predicted band size : 59 kDa

References for Anti-SIN1 antibody (ab116286)

ab116286 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"