Overview

  • Product name
  • Description
    Rabbit polyclonal to Sin3b
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region of Human Sin3b (NP_056075).

  • Positive control
    • Fetal brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab76231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 133 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

Target

  • Relevance
    Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • Histone deacetylase complex subunit Sin3b antibody
    • Paired amphipathic helix protein Sin3b antibody
    • SIN3 homolog B transcription regulator antibody
    • SIN3 transcription regulator family member B antibody
    • SIN3 transcription regulator homolog B antibody
    • Transcriptional corepressor Sin3b antibody
    see all

Images

  • Anti-Sin3b antibody (ab76231) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 133 kDa
    Observed band size : >168 kDa (why is the actual band size different from the predicted?)

References

ab76231 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab76231.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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