Overview

  • Product nameAnti-SIPA1 antibody
    See all SIPA1 primary antibodies
  • Description
    Rabbit polyclonal to SIPA1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to a region between residue 992 to 1042 of human SIPA1 (NP_006738.3).

  • Positive control
    • HeLa cells, 293T and mouse NIH3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab85928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 112 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
    • Tissue specificityExpressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
    • Sequence similaritiesContains 1 PDZ (DHR) domain.
      Contains 1 Rap-GAP domain.
    • Cellular localizationNucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the perinuclear membraneous region.
    • Information by UniProt
    • Database links
    • Alternative names
      • GTPase activating protein Spa 1 antibody
      • GTPase-activating protein Spa-1 antibody
      • MGC102688 antibody
      • MGC17037 antibody
      • p130 SPA-1 antibody
      • p130 SPA1 antibody
      • Signal induced proliferation associated 1 antibody
      • Signal induced proliferation associated gene 1 antibody
      • Signal induced proliferation associated protein 1 antibody
      • Signal-induced proliferation-associated protein 1 antibody
      • SIPA 1 antibody
      • Sipa-1 antibody
      • Sipa1 antibody
      • SIPA1_HUMAN antibody
      • Spa1 antibody
      see all

    Anti-SIPA1 antibody images

    • Detection of SIPA1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab85928 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
      Detection: Chemiluminescence with an exposure time of 30 seconds.
    • All lanes : Anti-SIPA1 antibody (ab85928) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : mouse NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 112 kDa
      Observed band size : 117 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

    References for Anti-SIPA1 antibody (ab85928)

    ab85928 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"