• Product name
  • Description
    Rabbit polyclonal to SIPA1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to a region between residue 992 to 1042 of human SIPA1 (NP_006738.3).

  • Positive control
    • HeLa cells, 293T and mouse NIH3T3 whole cell lysates



Our Abpromise guarantee covers the use of ab85928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 112 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
    • Tissue specificity
      Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.
    • Sequence similarities
      Contains 1 PDZ (DHR) domain.
      Contains 1 Rap-GAP domain.
    • Cellular localization
      Nucleus. Cytoplasm > perinuclear region. Endomembrane system. Mostly localized in the perinuclear membraneous region.
    • Information by UniProt
    • Database links
    • Alternative names
      • GTPase activating protein Spa 1 antibody
      • GTPase-activating protein Spa-1 antibody
      • MGC102688 antibody
      • MGC17037 antibody
      • p130 SPA-1 antibody
      • p130 SPA1 antibody
      • Signal induced proliferation associated 1 antibody
      • Signal induced proliferation associated gene 1 antibody
      • Signal induced proliferation associated protein 1 antibody
      • Signal-induced proliferation-associated protein 1 antibody
      • SIPA 1 antibody
      • Sipa-1 antibody
      • Sipa1 antibody
      • SIPA1_HUMAN antibody
      • Spa1 antibody
      see all

    Anti-SIPA1 antibody images

    • Detection of SIPA1 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab85928 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot.
      Detection: Chemiluminescence with an exposure time of 30 seconds.
    • All lanes : Anti-SIPA1 antibody (ab85928) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : mouse NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 112 kDa
      Observed band size : 117 kDa (why is the actual band size different from the predicted?)

      Exposure time : 3 minutes

    References for Anti-SIPA1 antibody (ab85928)

    ab85928 has not yet been referenced specifically in any publications.

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